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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.8
- 수록면
- 347 - 355 (9page)
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Purpose: Zinc is known to be associated with osteoblast proliferation and differentiation. Osterix as zinc-finger transcription factor is also related to osteoblast differentiation and bone formation. In the present study, we aimed to investigate whether zinc modulates osterix gene and protein expression in osteoblastic MC3T3-E1 cells.
Methods: MC3T3-E1 cells were cultured in zinc-dependent concentrations (0, 0.5, 1, 5, or 15 μM Zn), along with osteogenic control (normal osteogenic medium) for 1 and 3 days. The gene and protein expression levels of osterix were analyzed by real-time reverse transcription polymerase chain reaction and Western blotting, respectively.
Results: Zinc increased osteoblast proliferation in a concentration-dependent manner at day 1 and 3. Similarly, zinc increased the activity of osteoblast marker enzyme alkaline phosphatase in cells and media in a zinc concentration-dependent manner. Moreover, our results showed that the pattern of osterix gene expression by zinc was down-regulated within the low levels of zinc treatments (0.5–1 μM) at day 1, but it was up-regulated after extended culture period at day 3. Osterix protein expression by zinc showed the similar pattern of gene expression, which down-regulated by low zinc levels at day 1 and up-regulated back at day 3 as the early stage of osteoblast differentiation.
Conclusion: Our results suggest that zinc modulates osterix gene and protein expression in osteoblasts, particularly in low level of zinc at early stage of osteoblast differentiation period.
Methods: MC3T3-E1 cells were cultured in zinc-dependent concentrations (0, 0.5, 1, 5, or 15 μM Zn), along with osteogenic control (normal osteogenic medium) for 1 and 3 days. The gene and protein expression levels of osterix were analyzed by real-time reverse transcription polymerase chain reaction and Western blotting, respectively.
Results: Zinc increased osteoblast proliferation in a concentration-dependent manner at day 1 and 3. Similarly, zinc increased the activity of osteoblast marker enzyme alkaline phosphatase in cells and media in a zinc concentration-dependent manner. Moreover, our results showed that the pattern of osterix gene expression by zinc was down-regulated within the low levels of zinc treatments (0.5–1 μM) at day 1, but it was up-regulated after extended culture period at day 3. Osterix protein expression by zinc showed the similar pattern of gene expression, which down-regulated by low zinc levels at day 1 and up-regulated back at day 3 as the early stage of osteoblast differentiation.
Conclusion: Our results suggest that zinc modulates osterix gene and protein expression in osteoblasts, particularly in low level of zinc at early stage of osteoblast differentiation period.
목차
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
SUMMARY
REFERENCES
참고문헌 (25)
참고문헌 신청
Seibel MJ / 1992 / Urinary pyridinium crosslinks of collagen : specific markers of bone resorption in metabolic bone disease / Trends Endocrinol Metab 3(7):263~270
Aubin JE / 2002 / Principles of Bone Biology / Academic Press :59~81
Garnero P / 1996 / Increased bone turnover in late postmenopausal women is a major determinant of osteoporosis / J Bone Miner Res 11(3):337~349
Franceschi RT / 2007 / Transcriptional regulation of osteoblasts / Ann N Y Acad Sci 1116(1):196~207
Nakashima K / 2002 / The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation / Cell 108(1):17~29
Aubin JE / 2002 / Principles of Bone Biology / Academic Press :59~81
Garnero P / 1996 / Increased bone turnover in late postmenopausal women is a major determinant of osteoporosis / J Bone Miner Res 11(3):337~349
Franceschi RT / 2007 / Transcriptional regulation of osteoblasts / Ann N Y Acad Sci 1116(1):196~207
Nakashima K / 2002 / The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation / Cell 108(1):17~29
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UCI(KEPA) : I410-ECN-0101-2020-594-001155674