지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Single-dimensional (1-D) and two-dimensional (2-D) LC methods were utilized to separate peptides from various sources followed by MS/MS analysis. Two-dimensional ultra-high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than 1-D LC. The most popular 2-D LC approach used today for proteomic research combines strong cation exchange and reversed-phase LC. We have evaluated an alternative mode for 2-D LC of peptides using 2-D RP-RP nano UPLC Q-TOF Mass Spectrometry, employing reversed-phase columns in both separation dimensions. As control experiments, we identified 129 proteins in 1-D LC and 322 proteins in 2-D LC from E. coli extract peptides. Furthermore, we applied this method to rat primary hepatocyte and a total of 170 proteins were identified from 1-D LC, and 527 proteins were identified from all 2-D LC system. The in-depth protein profiling established by this 2-D LC MS/MS from rat primary hepatocyte could be a very useful reference for future applications in regards to drug induced liver toxicity.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Adkins, J. N / 2005 / A proteomic study of the HUPO Plasma Proteome Project's pilot samples using an accurate mass and time tag strategy / Proteomics 5:3454
Eriksson, J / 2005 / Protein identification in complex mixtures / J. Proteome. Res 4:387
Wolters, D. A / 2001 / An automated multidimensional protein identification technology for shotgun proteomics / Anal. Chem 73:5683
Shen, Y / 2005 / Automated 20 kpsi RPLC-MS and MS/MS with chromatographic peak capacities of 1000-1500 and capabilities in proteomics and metabolomics / Anal. Chem 77:3090
Echan, L. A / 2005 / Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma / Proteomics 5:3292
Eriksson, J / 2005 / Protein identification in complex mixtures / J. Proteome. Res 4:387
Wolters, D. A / 2001 / An automated multidimensional protein identification technology for shotgun proteomics / Anal. Chem 73:5683
Shen, Y / 2005 / Automated 20 kpsi RPLC-MS and MS/MS with chromatographic peak capacities of 1000-1500 and capabilities in proteomics and metabolomics / Anal. Chem 77:3090
Echan, L. A / 2005 / Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma / Proteomics 5:3292
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
임상검사실에서의 액체크로마토그래피-질량분석을 위한 권고안: 제3편. 질 보증
Laboratory Medicine Online
2020 .01
-
忍冬藤이 인간 유래 악성 흑색종 세포의 유전자 발현에 미치는 영향
한방안이비인후피부과학회지
2009 .01
-
열분무 LC / MS에 의한 인삼사포닌의 분석 ( Analysis of Ginsenosides by Thermospray LC / MS )
Journal of Ginseng Research
1995 .08
-
임상검사실에서의 액체크로마토그래피-질량분석을 위한 권고안: 제2편. 검사법 검증
Laboratory Medicine Online
2020 .01
-
Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories: a Questionnaire Survey
Annals of Laboratory Medicine
2019 .01
-
SPE-LC/MS/MS를 이용한 해수 중 잔류농약 다성분동시분석법 정립
환경독성보건학회 심포지엄 및 학술대회
2019 .04
-
A retrospective single-center study comparing clinical outcomes of 3-dimensional and 2-dimensional laparoscopic cholecystectomy in acute cholecystitis
Annals of Hepato-Biliary-Pancreatic Surgery
2019 .11
-
The prognoses and postoperative outcomes of patients with both colorectal cancer and liver cirrhosis based on a nationwide cohort in Korea
Annals of Surgical Treatment and Research
2020 .08
-
Posterior Shift of Contact Point between Femoral Component and Polyethylene in the LCS Rotating Platform Implant under Weight Bearing Condition
Knee Surgery and Related Research
2016 .01
-
Clinical Significance and Prognostic Value of Pentraxin-3 as Serologic Biomarker for Lung Cancer
Asian Pacific journal of cancer prevention : APJCP
2013 .01
-
A Comparison of the Clinical and Radiographic Results of Press Fit Condylar Rotating-Platform High-Flexion and Low Contact Stress Mobile Bearing Prosthesis in Total Knee Arthroplasty: Short term Results
Knee Surgery and Related Research
2012 .01
-
Optimization of Analytical Method for 1,2,3-Benzotriazole in aquatic toxicity test Using LC-MS/MS
환경독성보건학회 심포지엄 및 학술대회
2019 .10
-
Inhibitory Effect of Ginsenoside-Rp1, a Novel Ginsenoside Derivative, on the Functional Activation of Macrophage-like Cells
Biomolecules & Therapeutics(구 응용약물학회지)
2008 .01
-
Predictive Factors for Radiation Pneumonitis in Lung Cancer Treated with Helical Tomotherapy
Cancer Research and Treatment
2013 .01
-
Determination of oxytetracycline residues in eggs using LC-MS/MS
한국실험동물학회 학술발표대회 논문집
2020 .07
-
ELISA-LC/MS/MS 병행에 의한 식품 중 aflatoxins 분석
한국환경보건학회지
2010 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0