지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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An auxin-producing bacterium, Klebsiella michiganensis Jopap-1, was isolated from a button mushroom bed in Buyeo-Gun, Chungcheongnam-Do. The strain Jopap-1 was classified as a novel strain of K. michiganensis based on a chemotaxonomic and phylogenetic analysis. The isolated K. michiganensis Jopap-1 was confirmed to produce indole-3-acetic acid (IAA), which is one of auxin hormones by TLC and HPLC analyses. The maximum concentration of IAA ($96.05mg\;L^{-1}$) was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 48 h at $35^{\circ}C$ by HPLC quantity analysis. A negative relationship between IAA production and pH variation was estimated to show that the increase of IAA caused acidic pH in the culture. The effect of the supplement on L-tryptophan (precursor of IAA) production was observed to be highest at 0.1% concentration, but was significantly lowered above a concentration of 0.2%. To investigate the growth-promoting effects on the crops, the culture broth of E. michiganensis Jopap-1 was infected to water cultures and seed pots of mung bean and lettuce. Consequently, the adventitious root induction and root growth of mung bean and lettuce were observed to be 2.1 and 1.8 times higher than those of the control.
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참고문헌 (21)
참고문헌 신청
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Denvender KJ / 1984 / Characterization of a sub-stance produced by Azospirillum which causes branching of wheat root hairs / Can J Microbiol 31:206~210
Ezaki T / 1989 / Flu orometric deoxy-ribonucleic acid-deoxyribonucleic acid hybridization in micro- dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains / Int J System Bacteriol 39:224~229
Emmert EAB / 1999 / Biocontrol of plant disease: A gram-positive perspective / FEMS Microbiol Lett 171:1~9
Glick BR / 1999 / Biochemical and genetic mechani sms used by plant growth promoting bacteria / Imperial College Press :1~3
Denvender KJ / 1984 / Characterization of a sub-stance produced by Azospirillum which causes branching of wheat root hairs / Can J Microbiol 31:206~210
Ezaki T / 1989 / Flu orometric deoxy-ribonucleic acid-deoxyribonucleic acid hybridization in micro- dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains / Int J System Bacteriol 39:224~229
Emmert EAB / 1999 / Biocontrol of plant disease: A gram-positive perspective / FEMS Microbiol Lett 171:1~9
Glick BR / 1999 / Biochemical and genetic mechani sms used by plant growth promoting bacteria / Imperial College Press :1~3
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