Panduratin A Inhibits Cell Proliferation by Inducing G0/G1 Phase Cell Cycle Arrest and Induces Apoptosis in Breast Cancer Cells

Liu, Qiuming; Cao, Yali; Zhou, Ping; Gui, Shimin; Wu, Xiaobo; Xia, Yong; Tu, Jianhong

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저자정보: Liu, Qiuming (Department of Breast Surgery, Breast Cancer Institute, The Third Hospital of Nanchang) Cao, Yali (Department of Breast Surgery, Breast Cancer Institute, The Third Hospital of Nanchang) Zhou, Ping (Department of Breast Surgery, Breast Cancer Institute, The Third Hospital of Nanchang) Gui, Shimin (Department of Breast Surgery, Breast Cancer Institute, The Third Hospital of Nanchang) Wu, Xiaobo (Department of Breast Surgery, Breast Cancer Institute, The Third Hospital of Nanchang) Xia, Yong (Department of Breast Surgery, Breast Cancer Institute, The Third Hospital of Nanchang) Tu, Jianhong (Department of Breast Surgery, Breast Cancer Institute, The Third Hospital of Nanchang)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제26권 제3호

발행연도: 2018.1

수록면: 328 - 334 (7page)

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Because of the unsatisfactory treatment options for breast cancer (BC), there is a need to develop novel therapeutic approaches for this malignancy. One such strategy is chemotherapy using non-toxic dietary substances and botanical products. Studies have shown that Panduratin A (PA) possesses many health benefits, including anti-inflammatory, anti-bacterial, anti-oxidant and anticancer activities. In the present study, we provide evidence that PA treatment of MCF-7 BC cells resulted in a time- and dose-dependent inhibition of cell growth with an $IC_{50}$ of $15{\mu}M$ and no to little effect on normal human MCF-10A breast cells. To define the mechanism of these anti-proliferative effects of PA, we determined its effect critical molecular events known to regulate the cell cycle and apoptotic machinery. Immunofluorescence and flow cytometric analysis of Annexin V-FITC staining provided evidence for the induction of apoptosis. PA treatment of BC cells resulted in increased activity/expression of mitochondrial cytochrome C, caspases 7, 8 and 9 with a significant increase in the Bax:Bcl-2 ratio, suggesting the involvement of a mitochondrial-dependent apoptotic pathway. Furthermore, cell cycle analysis using flow cytometry showed that PA treatment of cells resulted in G0/G1 arrest in a dose-dependent manner. Immunoblot analysis data revealed that, in MCF-7 cell lines, PA treatment resulted in the dose-dependent (i) induction of $p21^{WAF1/Cip1}$ and p27Kip1, (ii) downregulation of Cyclin dependent kinase (CDK) 4 and (iii) decrease in cyclin D1. These findings suggest that PA may be an effective therapeutic agent against BC.

#Breast cancer #Panduratin A #Apoptosis #Cell cycle arrest #Cyclin D1

참고문헌 (33)

참고문헌 신청

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