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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 296 - 307 (12page)
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초록· 키워드
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In spite of frequent usage of primary human foreskin keratinocytes (HFKs) in the study of skin biology, senescence-induced block-age of in vitro proliferation has been a big hurdle for their effective utilization. In order to overcome this passage limitation, we first isolated ten HFK lines from circumcision patients and successfully immortalized four of them via a retroviral transduction of high-risk human papillomavirus (HPV) E6 and E7 oncogenes. We confirmed expression of a keratinocyte marker protein, keratin 14 and two viral oncoproteins in these immortalized HFKs. We also observed their robust responsiveness to various exogenous stimuli, which was evidenced by increased mRNA expression of epithelial differentiation markers and pro-inflammatory genes in response to three reactive chemicals. In addition, their applicability to cytotoxicity assessment turned out to be comparable to that of HaCaT cells. Finally, we confirmed their differentiation capacity by construction of well-stratified three dimensional skin cultures. These newly established immortalized HFKs will be valuable tools not only for generation of in vitro skin disease models but also for prediction of potential toxicities of various cosmetic chemicals.
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등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Allen-Hoffmann, B. L. / 2000 / Normal growth and differentiation in a spontaneously immortalized near-diploid human keratinocyte cell line, NIKS / J. Invest. Dermatol 114:444~455
Baden, H. P. / 1987 / Isolation and characterization of a spontaneously arising long-lived line of human keratinocytes (NM 1) / In Vitro Cell. Dev. Biol 23:205~213
Ben-Porath, I. / 2004 / When cells get stressed: an integrative view of cellular senescence / J. Clin. Invest 113:8~13
Ben-Porath, I. / 2005 / The signals and pathways activating cellular senescence / Int. J. Biochem. Cell Biol 37:961~976
Boukamp, P. / 1988 / Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line / J. Cell Biol 106:761~771
Baden, H. P. / 1987 / Isolation and characterization of a spontaneously arising long-lived line of human keratinocytes (NM 1) / In Vitro Cell. Dev. Biol 23:205~213
Ben-Porath, I. / 2004 / When cells get stressed: an integrative view of cellular senescence / J. Clin. Invest 113:8~13
Ben-Porath, I. / 2005 / The signals and pathways activating cellular senescence / Int. J. Biochem. Cell Biol 37:961~976
Boukamp, P. / 1988 / Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line / J. Cell Biol 106:761~771
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