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자료유형
학술저널
저자정보
Cheng, Jinhua (Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University) Jaiswal, Kumar Sagar (Center for Nutraceutical and Pharmaceutical Materials, Myongji University) Yang, Seung Hwan (Center for Nutraceutical and Pharmaceutical Materials, Myongji University) Suh, Joo-Won (Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제59권 제2호
발행연도
2016.1
수록면
149 - 154 (6page)

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An endophytic bacterial strain was isolated from kimchi, a Korean traditional fermented Brassica campestris and identified as Bacillus subtilis MJMP2 based on the 16S rRNA sequence. This strain showed strong antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331, the pathogen of bacterial rice blight disease, as well as activity against some other rice phytopathogenic fungi. The active compound was purified through size-exclusion chromatography and preparative High-performance liquid chromatography. The molecular weight was determined as m/z 1043 by mass spectroscopy, which is identical to that of iturin A. Furthermore, a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay. The crude extract also enhanced the length of roots of Arabidopsis thaliana. These results suggest that the strain Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight disease.

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