Human Papillomavirus E6 Knockdown Restores Adenovirus Mediated-estrogen Response Element Linked p53 Gene Transfer in HeLa Cells

Kajitani, Koji; Ken-Ichi, Honda; Terada, Hiroyuki; Yasui, Tomoyo; Sumi, Toshiyuki; Koyama, Masayasu; Ishiko, Osamu

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자료유형: 학술저널

저자정보: Kajitani, Koji (Department of Obstetrics and Gynecology, Osaka City General Hospital) Ken-Ichi, Honda (Department of Obstetrics and Gynecology, Kashiwara Municipal Hospital) Terada, Hiroyuki (Department of Obstetrics and Gynecology, Kashiwara Municipal Hospital) Yasui, Tomoyo (Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine) Sumi, Toshiyuki (Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine) Koyama, Masayasu (Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine) Ishiko, Osamu (Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제18호

발행연도: 2015.1

수록면: 8,239 - 8,245 (7page)

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The p53 gene is inactivated by the human papillomavirus (HPV) E6 protein in the majority of cervical cancers. Treatment of HeLa S3 cells with siRNA for HPV E6 permitted adenovirus-mediated transduction of a p53 gene linked to an upstream estrogen response element (ERE). Our previous study in non-siRNA treated HHUA cells, which are derived from an endometrial cancer and express estrogen receptor ${\beta}$, showed enhancing effects of an upstream ERE on adenovirus-mediated p53 gene transduction. In HeLa S3 cells treated with siRNA for HPV E6, adenovirus-mediated transduction was enhanced by an upstream ERE linked to a p53 gene carrying a proline variant at codon 72, but not for a p53 gene with arginine variant at codon 72. Expression levels of p53 mRNA and Coxsackie/adenovirus receptor (CAR) mRNA after adenovirus-mediated transfer of an ERE-linked p53 gene (proline variant at codon 72) were higher compared with those after non-ERE-linked p53 gene transfer in siRNA-treated HeLa S3 cells. Western blot analysis showed lower ${\beta}$-tubulin levels and comparatively higher p53/${\beta}$-tubulin or CAR/${\beta}$-tubulin ratios in siRNA-treated HeLa S3 cells after adenovirus-mediated ERE-linked p53 gene (proline variant at codon 72) transfer compared with those in non-siRNA-treated cells. Apoptosis, as measured by annexin V binding, was higher after adenovirus-mediated ERE-linked p53 gene (proline variant at codon 72) transfer compared with that after non-ERE-linked p53 gene transfer in siRNA-treated cells.

#Adenovirus mediated transduction #codon 72 polymorphism #p53 #siRNA

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