MiR-454 Prompts Cell Proliferation of Human Colorectal Cancer Cells by Repressing CYLD Expression

Liang, Hong-Liang; Hu, Ai-Ping; Li, Sen-Lin; Xie, Jia-Ping; Ma, Qing-Zhu; Liu, Ji-Yong

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자료유형: 학술저널

저자정보: Liang, Hong-Liang (Department of Gastroenterology, Shandong Provincial Hospital, Shandong University) Hu, Ai-Ping (Department of Oncology, Liaocheng Tumor Hospital) Li, Sen-Lin (Department of Gastroenterology, Liaocheng People's Hospital) Xie, Jia-Ping (Department of Gastroenterology, Liaocheng People's Hospital) Ma, Qing-Zhu (Department of Gastroenterology, Liaocheng People's Hospital) Liu, Ji-Yong (Department of Gastroenterology, Shandong Provincial Hospital, Shandong University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제6호

발행연도: 2015.1

수록면: 2,397 - 2,402 (6page)

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Previous studies have shown that miR-454 plays an important role in a variety of biological processes in various human cancer cells. However, the underlying mechanisms of this microRNA in colorectal cancer (CRC) cells remain largely unknown. In the present study, we investigated the miR-454 role in CRC cell proliferation. We found that miR-454 expression is markedly upregulated in CRC tissues and CRC cells compared with the matched tumor adjacent tissues and the FHC normal colonic cell line. Ectopic expression of miR-454 promoted the proliferation and anchorage-independent growth of CRC cells, whereas inhibition of miR-454 reduced this effect. Bioinformatics analysis further revealed cylindromatosis (CYLD), a putative tumor suppressor as a potential target of miR-454. Data from luciferase reporter assays showed that miR-454 directly binds to the 3'-untranslated region (3'-UTR) of CYLD mRNA and repressed expression at both transcriptional and translational levels. In functional assays, CYLD-silenced in miR-454-in-transfected SW480 cells have positive effect to promote cell proliferation, suggesting that direct CYLD downregulation is required for miR-454-induced CRC cell proliferation. In sum, our data provide compelling evidence that miR-454 functions as an onco-miRNA, playing a crucial role in the promoting cell proliferation in CRC, and its oncogenic effect is mediated chiefly through direct suppression of CYLD expression.

#miR-454 #colorectal cancer #CYLD #cell proliferation

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