Application of chloroplast promoters of Cyanidioschyzon merolae for exogenous protein expression

Krupnik, Tomasz; Wasilewska, Wioleta; Drozak, Anna; Romanowska, Elzbieta; Zienkiewicz, Maksymilian

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자료유형: 학술저널

저자정보: Krupnik, Tomasz (Faculty of Biology, University of Warsaw) Wasilewska, Wioleta (Faculty of Biology, University of Warsaw) Drozak, Anna (Faculty of Biology, University of Warsaw) Romanowska, Elzbieta (Faculty of Biology, University of Warsaw) Zienkiewicz, Maksymilian (Faculty of Biology, University of Warsaw)

저널정보: 한국조류학회(藻類) Algae Algae 제33권 제4호

발행연도: 2018.1

수록면: 351 - 358 (8page)

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The ability to transform the chloroplast of Cyanidioschyzon merolae was limited by lack of confirmed and reliable promoter sequences (among other reasons), capable of delivering stable or modulated DNA transcription followed by protein synthesis. Our research has confirmed the applicability of three selected chloroplast promoters in C. merolae chloroplast overexpression of the exogenous protein (i.e., chloramphenicol acetyltransferase) and genetic transformation. These results might facilitate further research on genetically modified strains of C. merolae to envisage yet unknown aspect of cellular and plastic physiology as well as C. merolae potential applications as bio-factories or sources of useful chemicals.

#chloroplast promoters #chloroplast transformation #Cyanidioschyzon merolae

참고문헌 (18)

참고문헌 신청

Bock, R. / 2007 / Plastid biotechnology: prospects for herbicide and insect resistance, metabolic engineering and molecular farming / Curr Opin Biotechnol 18:100~106

Daniel, E. / 2015 / ATGme: Open-source web application for rare codon identification and custom DNA sequence optimization / BMC Bioinformatics 16:303

Daniell, H. / 2009 / Plant-made vaccine antigens and biopharmaceuticals / Trends Plant Sci 14:669~679

Hanahan, D. / 1983 / Studies on transformation of Escherichia coli with plasmids / J Mol Biol 166:557~580

Kanesaki, Y. / 2012 / External light conditions and internal cell cycle phases coordinate accumulation of chloroplast and mitochondrial transcripts in the red alga Cyanidioschyzon merolae / DNA Res 19:289~303

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