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자료유형
학술저널
저자정보
Woo, Seon-Ock (South Sea Environment Research Department, Korea Ocean Research and Development Institute) Jeon, Hye-Young (South Sea Environment Research Department, Korea Ocean Research and Development Institute) Lee, Jong-Rak (Laboratory of Marine Biodiversity, In The Sea Korea Co., Ltd.) Song, Jun-Im (Department of Biological Science, College of Natural Sciences, Ewha Womans University) Park, Hong-Seog (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) Yum, Seung-Shic (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
저널정보
대한독성유전단백체학회 Molecular & cellular toxicology Molecular & cellular toxicology 제6권 제4호
발행연도
2010.1
수록면
387 - 393 (7page)

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The extensive isolation of genes responsive to hyperthermal stress conditions in soft coral (Scleronephthya gracillimum) is described. Soft coral colonies were exposed to high seawater temperature conditions. Gene candidates whose transcript levels changed in response to hyperthermal conditions were identified by differential display polymerase chain reaction (DD-PCR). Twenty-four types of candidate genes were identified, 18 of which were upregulated in expression and 6 of which were downregulated. The genes were found to function in post-translational modification, protein turnover and chaperones (O); translation, ribosomal structure and biogenesis (J); signal transduction mechanisms (T); defense mechanisms (V); inorganic ion transport and metabolism (P); energy production and conversion (C); cytoskeleton (Z); cell cycle control, cell division and chromosome partitioning (D); lipid transport and metabolism (I); chromatin structure and dynamics (B); transcription (K); replication, recombination and repair (L); secondary metabolites biosynthesis, transport and catabolism (Q); extracellular structures (W); general function prediction (R); and finally, unknown function (S) based on KOG classification. Among these candidates, their expressional changes were confirmed by real-time quantitative PCR (qRT-PCR). These 24 isolated gene candidates were differentially expressed and therefore have great potential as molecular biomarkers for the identification of environmental stressors.

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