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논문 기본 정보

자료유형
학술저널
저자정보
Cho, Sang-Buem (Department of Animal Science and Environment, Konkuk University) Chang, Woo-Kyung (Department of Animal Science and Environment, Konkuk University) Kim, Yun-Jung (Department of Animal Science and Environment, Konkuk University) Moon, Hyung-In (Department of Animal Science and Environment, Konkuk University) Joo, Jong-Won (Department of Companion Animal Science, Joongbu University) Choi, In-Soo (College of Veterinary Medicine, Konkuk University) Seo, Kun-Ho (College of Veterinary Medicine, Konkuk University) Kim, Soo-Ki (Department of Animal Science and Environment, Konkuk University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제30권 제5호
발행연도
2010.1
수록면
764 - 772 (9page)

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Staphylococcus aureus lipase is regarded as a virulence factor. The response of lipase activity to various factors can provide important insights concerning the prevention of S. aureus during meat fermentation. This study was conducted to evaluate the main effects of nutrients used in culture media, and their combined effects on the inhibition of lipase activity and cell growth of pathogenic S. aureus SK1593 isolated from fermented pork meat. A Plackett-Burman design was used to evaluate the main effects of variables, including olive oil, soybean oil, grapeseed oil, sesame oil, $CuSO_4$, $MgCl_2$, $KNO_3$, $CaCl_2$, and KCl. Significant negative effects on lipase activity were detected with soybean oil, grapeseed oil, $KNO_3$, and $CaCl_2$. Additionally, these nutrients were further selected as variables for the investigation of their combined effect on lipase activity, via response surface methodology. In order to confirm the regression model, a situation that only inhibits lipase activity was simulated. The predicted lipase activity and cell growth of the simulated situation were 14.0 U/mL and $9.6\;{\log}_{10}$ (CFU/mL), respectively, and the estimated value of those in the same medium showed 15.14 U/mL and $9.4\;{\log}_{10}$(CFU/mL) respectively. The lipase activity of the simulated medium was inhibited approximately 5-fold as compared to the basal medium, but no significant differences in cell counts were noted to exist between the basal and simulated media. These results suggest that soybean oil, grapeseed oil, $KNO_3$, and $CaCl_2$ can be used to inhibit the growth of pathogenic S. aureus during the process of meat fermentation.

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