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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 175 - 180 (6page)
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초록· 키워드
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The objectives of this study were: i) to detect the presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Twenty (12.5%) of the samples were found to be contaminated with S. aureus. A total of 40 isolates from the 20 positive samples were confirmed to be S. aureus by multiplex PCR based on 16S rRNA and nuc gene. The mec A gene was not detected in any of the S. aureus strains. In the present study, 39 out of 40 (97.5%) isolates were found to be resistant to one or more antibiotics. All of isolates were susceptible to gentamicin, oxacillin, and vancomycin. The highest resistance rate was detected in penicillin (95%) and ampicillin (92.5%), followed by tetracycline (30%), erythromycin (20%), ciprofloxacin (12.5%). Nine major patterns were determined by PFGE. In 6 of these patterns, thirty-six strains (90%) had identical PFGE profiles.
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참고문헌 (28)
참고문헌 신청
Bennett, R. W. / 1998 / FDA’s Bacteriological Analytical Manual
Brakstad, O. G. / 1992 / Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene / J. Clin. Microbiol 30:1654~1660
Can, H. Y. / 2012 / Detection of enterotoxigenic and antimicrobial resistant S. aureus in Turkish cheeses / Food Control 24:100~103
Clinical and Laboratory Standards Institute / 2013 / Performance standards for antimicrobial susceptibility testing;twenty-third informational supplement
Crago, B. / 2010 / Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in food samples associated with foodborne illness in Alberta, Canada from 2007 to 2010 / Food Microbiol 32:202~205
Brakstad, O. G. / 1992 / Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene / J. Clin. Microbiol 30:1654~1660
Can, H. Y. / 2012 / Detection of enterotoxigenic and antimicrobial resistant S. aureus in Turkish cheeses / Food Control 24:100~103
Clinical and Laboratory Standards Institute / 2013 / Performance standards for antimicrobial susceptibility testing;twenty-third informational supplement
Crago, B. / 2010 / Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in food samples associated with foodborne illness in Alberta, Canada from 2007 to 2010 / Food Microbiol 32:202~205
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