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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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This study was conducted to examine the effect of egg shell membrane hydrolysates (ESMH) on wrinkle, UV, and moisture protection for cosmetic use. ESMH were fragmented as whole ESMH (before fractioning), Fraction I (> 10 kDa), Fraction II (3-10 kDa), and Fraction III (< 3 kDa). In order to test whether fractionated ESMH can be used for functional cosmetic materials, we examined not only the level of hyaluronic acid and collagen production, but also the MMP-1 activity using a HaCaT and CCD-986Sk cell line. Our study treated each sample of fractionated ESMH with different concentrations (0.01, 0.1, 1 mg/mL). In our in vivo research, we used hairless mice that had been exposed to UV-B to induce wrinkles for 7 wk, then applied Fraction I to the treatment group for 5 wk and then tested skin thickness, minimum erythema dose and moisture content. In addition, Fraction I was high in collagen and HA biosynthesis and it was better than TGF-${\beta}$ in improving of the skin. When TNF-${\alpha}$ caused MMP-1 activity in the CCD-986Sk cells, the whole ESMH and Fraction I proved to be effective in hindering the induction of collagenase depending on the concentration, and also showed outstanding effects in the suppression of skin aging. We found that the treatment group mice's UV-B radiation-induced skin damage was largely mitigated compared to that of the non-treatment group mice. Thus, we have concluded that EMSH helps to mitigate UV-B radiation-induced wrinkles, collagen, HA, MMP-1 activity and can be used for functional cosmetic materials.
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참고문헌 (38)
참고문헌 신청
Bae, J. Y. / 2009 / Bog blueberry anthocyanins alleviate photoaging in UV-B irradiation-induced human dermal fibroblasts / Mol. Nutr. Food Res 53:726~738
Candilish, J. K. / 1969 / L-5-hydroxylysine as aconstituent of the shell membrane of the hen’s egg / Int. J. Protein Res 1:299~302
Chisato, O. / 2013 / Collagen hydrolysate intake improves the loss of epidermal barrier function and skin elasticity induced by UVB irradiation in hairless mice / Photodermatol. Photoimmunol. Photomed 29:204~211
Cho, H. S. / 2007 / Anti-wrinkling effects of the mixture of vitamin C, vitamin E, pycnogenol and evening primrose oil, and molecular mechanisms on hairless mouse skin caused by chronic UV B irradiation / Photodermatol. Photoimmunol. Photomed 23:155~162
Chung J. H. / 2003 / Photoaging in Asians / Photodermatol. Photonimmunol. Photomed 19:109~121
Candilish, J. K. / 1969 / L-5-hydroxylysine as aconstituent of the shell membrane of the hen’s egg / Int. J. Protein Res 1:299~302
Chisato, O. / 2013 / Collagen hydrolysate intake improves the loss of epidermal barrier function and skin elasticity induced by UVB irradiation in hairless mice / Photodermatol. Photoimmunol. Photomed 29:204~211
Cho, H. S. / 2007 / Anti-wrinkling effects of the mixture of vitamin C, vitamin E, pycnogenol and evening primrose oil, and molecular mechanisms on hairless mouse skin caused by chronic UV B irradiation / Photodermatol. Photoimmunol. Photomed 23:155~162
Chung J. H. / 2003 / Photoaging in Asians / Photodermatol. Photonimmunol. Photomed 19:109~121
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