지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objective: The purpose of this study was to evaluate the efficacy and effect of various cryopreservation method on the survival and the cytoskeletal stability of metaphase II mouse oocyte. Methods: Mouse ovulated oocytes were collected and cryopreserved by a modified slow-freezing method with 1.5 M 1, 2-propanediol (PrOH)+0.1 M sucrose or by vitrification using cryo loop and EM grid with 40% ethylene glycol+0.6 M sucrose. Four hours after thawing, intact oocytes were fixed and stained with fluorescein isothiocyanate (FITC)-conjugated monoclonal anti-$\beta$-tubulin antibody to visualize spindle and propidium iodide (PI) to visualize chromosome. Spindle morphology was classified as follows: normal (barrel-shaped), slightly and absolute abnormal (multipolar or absent). Results: Survival rate of the frozen-thawed oocytes in vitrification group was significantly higher than that of slow-freezing group (62.7% vs. 24.4%, p<0.01). Vitrification with cryo loop showed significantly higher survival rate than that with EM grid (67.7% vs. 53.5%, p<0.05). On the other hand, proportion of normal spindle and chromosome configurations of the frozen-thawed oocytes between two vitrification group was not significantly different. Conclusion: For mouse ovulated oocytes, vitrification with cryo loop may be a preferable procedure compared to slow-freezing method. Further study should be needed to investigate developmental competency of frozen-thawed mouse oocytes.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
생쥐난자의 성숙단계와 결빙억제제에 따른 동결 및 해빙 후 생존율에 관한 연구
대한불임학회잡지
1991 .01
-
생쥐 난자의 초자화동결 시 Cytochalasin B가 Cytoskeleton에 미치는 영향
대한불임학회지
2002 .01
-
동결 전 단계적 노출처리방법이 유리화동결 및 초급속동결-융해 후 생쥐 성숙난자의 생존력에 미치는 영향에 관한 연구
대한불임학회지
2000 .01
-
Clinical Application of Human Oocyte Vitrification
대한생식의학회 학술대회
2006 .01
-
The Cytoskeletal and Chromosomal Constitution of Vitrified Immature Mouse Oocytes
대한불임학회잡지
1999 .01
-
Oocyte Freezing using Vitrification;Our Experience with fertile population
대한생식의학회 학술대회
2006 .01
-
인간의 미성숙난자의 동결보존에 관한 연구
대한불임학회잡지
1992 .01
-
The Cytoskeletal and Chromosomal Constitution in Vitrified Mouse Oocyte
대한불임학회잡지
1998 .01
-
Development of Effective Cryopreservation Method for Mouse Oocytes
대한생식의학회 학술대회
2003 .01
-
Effects of various combinations of cryoprotectants and cooling speed on the survival and further development of mouse oocytes after vitrification
Clinical and experimental reproductive medicine : CERM
2011 .01
-
Simplified EM Grid Vitrification Is a Convenient and Efficient Method for Mouse Mature Oocyte Cryopreservation
Yonsei Medical Journal
2006 .01
-
Oocyte Quality;What does it mean and What determines it ?
대한생식의학회 학술대회
1996 .01
-
Super-Rapid Cooling Using Slush Nitrogen ($SN_2$) Increases the Efficacy of Human Oocytes Vitrification and Subsequent Clinical Applications
대한생식의학회 학술대회
2006 .01
-
Developmental competence of in vitro matured porcine oocyte after vitrification and warming
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Vitrification of Human Oocytes at Various Stage of Maturation
대한생식의학회 학술대회
2004 .01
-
Ongoing Pregnancy after Vitrification of Human Oocytes
대한생식의학회 학술대회
2007 .01
-
Cumulus Free 생쥐 성숙란의 초자화 동결-융해 후 Simple Media에서의 수정 및 배 발달
대한불임학회지
2002 .01
-
햄스터 난자의 동결보존과 그의 임상적 이용에 관한 연구
대한불임학회잡지
1991 .01
-
인간 미성숙난자의 동결.융해후 체외 배양된 난자에 대한 염색체 분석
대한불임학회잡지
1997 .01
-
$In$ $vitro$ development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification
Clinical and experimental reproductive medicine : CERM
2011 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0