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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Mi-Ok (Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University) Hwang, Ji-Hye (Department of Life Science, Ewha Womans University) Lee, Dong-Hee (Department of Life Science, Ewha Womans University) Hong, Choo-Bong (Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University)
저널정보
한국식물학회 식물학회지 식물학회지 제50권 제4호
발행연도
2007.1
수록면
496 - 503 (8page)

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Although flooding can often severely damage crop yields, few studies of this stress have been made at the genetic level. To identify the genes that probably function in plants at the onset of flooding stress, we constructed a cDNA library representing tobacco plants that experienced short-term stress, i.e., 2 to 4 h of submergence while under illumination. Differential screen ing of that library produced 73 cDNA clones that showed preferential hybridization with the probe prepared from these stressed plants. The cDNA inserts were isolated from the vector by restriction digest and subjected to reverse northern analysis, which confirmed preferential expression of 41 genes. The remainder either had no significant increase in expression under flooding stress or exhibited no identifiable signal. We then performed northern blot analyses for some selected genes to provide supporting evidence that strongly paralleled our results from the reverse-northern evaluation. Photosynthesis related genes were the major group, followed by those for well-known glycolysis enzymes and fermentation enzymes. Other genes include those for hydrolytic enzymes and components of the ethylene synthesis pathway. Although many others also were induced, their functions could not be characterized here.

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