지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Capillary electrophoresis (CE) is an effective technique to study chiral recognition because it offers flexibility in adjusting vital factors. Currently, various available cyclodextrins (CDs) can be employed for the chiral separation of numerous analytes. Herein, we investigate the enantioseparation behavior of lipoic acid enantiomers in various types of single and dual CD systems through CE. Additionally, several impacted CE parameters were optimized through the systematic investigation based on the design of experiment (DoE) concept for a single system comprising a heptakis (2,3,6-tri-O-methyl)-β-CD and a dual system containing the combination of the single CD with a sulfated-β-CD. Consequently, absolute enantioresolution was obtained within 15 min on a common standard bare fused-silica capillary (64.5/56 cm in total/effective length, 50/365 μm inner/outer diameter), maintained at 15℃ and at an applied voltage of 24 kV. The optimal background electrolyte consisted of 6 mM heptakis (2,3,6-tri-O-methyl)-β-CD dissolved in the solution of 58 mM borate buffer at pH 10. Furthermore, the results of apparent binding constant experiments indicated that the Senantiomer-heptakis (2,3,6-tri-O-methyl)-β-CD complex exhibited a stronger affinity than its R-enantiomer counterpart. The obtained electrophoretic mobility values could be utilized to interpret the resolution achieved at various CD concentrations and the mobility behavior of the complexes elucidated the migration order of the enantiomers in an electropherogram.
목차
Abstract
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusions
References
참고문헌 (21)
참고문헌 신청
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A. R. Smith, S. V. Shenvi, M. Widlansky, J. H. Suh and T. M. Hagen, Curr. Med. Chem., 11(9), 1135-1146 (2004).
W. Jones, X. Li, Zhi-chao Qu, L. Perriott, R. R. Whitesell and J. M. May, Free Radical Biology and Medicine, 33(1), 83-93 (2002).
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B. Salehi, Y. Berkay Yılmaz, G. Antika, T. Boyunegmez Tumer, M. Fawzi Mahomoodally, D. Lobine, M. Akram, M. Riaz, E. Capanoglu, F. Sharopov, N. Martins, W. C. Cho and J. Sharifi-Rad, Biomolecules, 9(8), 356 (2019).
A. R. Smith, S. V. Shenvi, M. Widlansky, J. H. Suh and T. M. Hagen, Curr. Med. Chem., 11(9), 1135-1146 (2004).
W. Jones, X. Li, Zhi-chao Qu, L. Perriott, R. R. Whitesell and J. M. May, Free Radical Biology and Medicine, 33(1), 83-93 (2002).
J. H. Suh, R. Moreau, S. H. Heath and T. M. Hagen, Redox Rep., 10(1), 52-60 (2005).
B. Salehi, Y. Berkay Yılmaz, G. Antika, T. Boyunegmez Tumer, M. Fawzi Mahomoodally, D. Lobine, M. Akram, M. Riaz, E. Capanoglu, F. Sharopov, N. Martins, W. C. Cho and J. Sharifi-Rad, Biomolecules, 9(8), 356 (2019).
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