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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 205 - 205 (1page)
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Necroptosis is one of the regulated cell death pathways, which shares several upstream signaling elements with apoptosis. It and can be triggered by tumor necrosis factor α (TNFα) and other death ligands. Activation of necroptosis involves sequential actions of cytoplasmic effectors, receptor-interacting serine/threonine-protein kinase 1 (RIPK1), RIPK3, and mixed lineage kinase domain-like (MLKL). Necroptosis leads to plasma membrane rupture and release of danger associated molecular patterns (DAMPS) which includes various inflammatory chemokines. In this study, we examined whether major necroptosis effectors are expressed in mouse oocytes and whether oocytes respond to necroptosis-inducing signal. We examined expression of RIPK1, RIPK3, and MLKL in mouse oocytes by RT-PCR and immunofluorescence staining. All three factors are expressed in germinal vesicle (GV) and polar body (PB) stage oocytes. RIPK3 and MLKL are localized within the nucleus of GV oocytes when the nuclear membrane is intact, but do not sh ... 전체 초록 보기
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