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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 229 - 229 (1page)
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Autophagy is well-conserved cellular recycling process. However, autophagy in in-vitro porcine oocyte maturation have not been discovered yet. In this study, we confirmed autophagosome in-vitro culturing porcine oocytes by detection of LC3 which is promised marker protein of autophagosome. Then, we also examine the relationship between autophagy and oocyte maturation by treatment of autophagic inhibitors or nuclear maturation inhibitors. Autophagy activation was regarded to amount of autophagosome in this study. To autophagosome detection in porcine oocytes, Immunochemistry and westernblotting using LC3 antibody was conducted by culturing time of 0 h, 14 h, 28 h, and 42 h. Then, we measured LC3-II levels using western blotting after inhibiting nuclear maturation of oocytes via cAMP treatment in an in vitro culture, to clarify whether nuclear maturation affects autophagy. To clarify whether autophagy affects nuclear maturation, we also counted mature oocytes after inhibiting ... 전체 초록 보기
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