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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 209 - 217 (9page)
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The presence of high amounts of seed storage proteins (SSPs) improves the overall quality of soybean seeds. However, these SSPs pose a major limitation due to their high abundance in soybean seeds. Although various technical advancements including mass-spectrometry and bioinformatics resources were reported, only limited information has been derived to date on soybean seeds at proteome level. Here, we applied a tandem mass tags (TMT)-based quantitative proteomic analysis to identify the significantly modulated proteins in the seeds of two soybean cultivars showing varying protein contents. This approach led to the identification of 5,678 proteins of which 13 and 1,133 proteins showed significant changes in Daewon (low-protein content cultivar) and Saedanbaek (high-protein content cultivar) respectively. Functional annotation revealed that proteins with increased abundance in Saedanbaek were mainly associated with the amino acid and protein metabolism involved in protein synthesis, folding, targeting, and degradation. Taken together, the results presented here provide a pipeline for soybean seed proteome analysis and contribute a better understanding of proteomic changes that may lead to alteration in the protein contents in soybean seeds.
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참고문헌 (42)
참고문헌 신청
Badole SL / 2015 / Glucose intake and utilization in pre-diabetes and diabetes: Implications for cardiovascular disease / Academic Press :225~227
Boersema PJ / 2015 / Proteomics beyond large-scale protein expression analysis / Curr. Opin. Biotechnol 34:162~170
Fait A / 2006 / Arabidopsis seed development and germination is associated with temporally distinct metabolic switches / Plant Physiol 142:839~854
Galili G / 2014 / The role of photosynthesis and amino acid metabolism in the energy status during seed development / Front. Plant Sci 5:1~6
Gallardo K / 2007 / A combined proteome and ranscriptome analysis of developing Medicago truncatula seeds: Evidence for metabolic specialization of maternal and filial tissues / Mol. Cell. Proteomics 6:2165~2179
Boersema PJ / 2015 / Proteomics beyond large-scale protein expression analysis / Curr. Opin. Biotechnol 34:162~170
Fait A / 2006 / Arabidopsis seed development and germination is associated with temporally distinct metabolic switches / Plant Physiol 142:839~854
Galili G / 2014 / The role of photosynthesis and amino acid metabolism in the energy status during seed development / Front. Plant Sci 5:1~6
Gallardo K / 2007 / A combined proteome and ranscriptome analysis of developing Medicago truncatula seeds: Evidence for metabolic specialization of maternal and filial tissues / Mol. Cell. Proteomics 6:2165~2179
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