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논문 기본 정보

자료유형
학술저널
저자정보
Bir Bahadur Thapa (Annapurna Research Center Kathmandu) Laxmi Sen Thakuri (Annapurna Research Center Kathmandu) Pusp Raj Joshi (Annapurna Research Center Kathmandu) Krishna Chand (Annapurna Research Center Kathmandu) Sabari Rajbahak (Annapurna Research Center Kathmandu) Anil Kumar Sah (Annapurna Research Center Kathmandu) Resha Shrestha (Annapurna Research Center Kathmandu) Mukti Ram Paudel (Annapurna Research Center Kathmandu) 박소영 (충북대학교) Bijaya Pant (Annapurna Research Center Kathmandu)
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제47권 제4호
발행연도
2020.1
수록면
330 - 336 (7page)

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Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.

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