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논문 기본 정보

자료유형
학술저널
저자정보
Kim Young Ah (Department of Laboratory Medicine National Health Insurance Service Ilsan Hospital Goyang Korea) Kim Hyunsoo (Department of Laboratory Medicine National Police Hospital Seoul Korea) Seo Young Hee (Research Institute of Bacterial Resistance Yonsei University College of Medicine Seoul Korea) Park Go Eun (Research Institute of Bacterial Resistance Yonsei University College of Medicine Seoul Korea) Lee Hyukmin (Research Institute of Bacterial Resistance Yonsei University College of Medicine Seoul KoreaDepartm) Lee Kyungwon (Research Institute of Bacterial Resistance Yonsei University College of Medicine Seoul KoreaDepartm)
저널정보
대한진단검사의학회 Annals of Laboratory Medicine Annals of Laboratory Medicine 제41권 제3호
발행연도
2021.1
수록면
285 - 292 (8page)

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Background: One health is a flexible concept with many facets, including the environment, community, and the nosocomial super-bacteria resistance network. We investigated the molecular prevalence of extended-spectrum-β-lactamase-producing Escherichia coli (ESBL-EC) in workers, livestock, and the farm environment in Korea. Methods: ESBL-EC isolates were obtained from samples from 19 swine farms, 35 retail stores, seven slaughterhouses, and 45 related workers throughout Korea from August 2017 to July 2018, using ChromID ESBL (BioMerieux, Marcy l’Etoile, France) agar and enrichment broth. The presence of ESBL and mobilized colistin resistance (mcr) genes and antimicrobial resistance were determined. Clonality was evaluated with pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results: In total, 232 ESBL-EC isolates were obtained from 1,614 non-duplicated samples (14.4% positive rate). The ESBL-EC isolates showed regional and source-related differences. blaCTX-M-55 (N=100), blaCTX-M-14 (N=65), blaCTX-M-15 (N=33), and blaCTX-M-65 (N=23) were common ESBL types. The ESBL-EC isolates showed high resistance rates for various antimicrobial classes; however, all isolates were susceptible to carbapenem. One swine-originating colistin-resistant isolate did not carry any known mcr gene. PFGE was successful for 197 of the 232 isolates, and most PFGE types were heterogeneous, except for some dominant PFGE types (O, R, T, U, and V). MLST of 88 isolates was performed for representative PFGE types; however, no dominant sequence type was observed. Conclusions: The proportion of ESBL-EC in swine industry-related samples was significant, and the isolates harbored common clinical ESBL gene types. These molecular epidemiologic data could provide important evidence for antimicrobial-resistance control through a one health approach.

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