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Background: The leaves of Perilla frutescens, commonly called perilla and used for food in Korea, contain components with a variety of biological effects and potential therapeutic applications. The purpose of this study was to identify the components of 70% ethanol extracted Perilla frutescens (EEPF) and determine its inhibitory effects on oral microbial activity and production of nitric oxide (NO) and prostaglandin E2 (PGE2) in lipopolysaccharides (LPS)-stimulated Raw264.7 macrophages, consequently, to confirm the possibility of using EEPF as a functional component for improving the oral environment and preventing inflammation. Methods: One kg of P. frutescens leaves was extracted with 70% ethanol and dried at ?70oC. EEPF was analyzed using high-performance liquid chromatography analysis, and antimicrobial activity against oral microorganisms was revealed using the disk diffusion test. Cell viability was elucidated using a methylthiazolydiphenyl-tetrazolium bromide assay, and the effect of EEPF on LPS-induced morphological variation was confirmed through microscopic observation. The effect of EEPF on LPS-induced production of pro-inflammatory mediators, NO and PGE2 was confirmed by the NO assay and PGE2 enzyme-linked immunosorbent assay. Results: The main component of EEPF was rosemarinic acid, and EEPF showed weak anti-bacterial and anti-fungal effects against microorganisms living in the oral cavity. EEPF did not show toxicity to Raw264.7 macrophages and had inhibitory effects on the morphological variations and production of pro-inflammatory mediators, NO and PGE2 in LPS-stimulated Raw264.7 macrophages. Conclusion: EEPF can be used as a functional material for improving the oral environment through the control of oral microorganisms and for modulating inflammation by inhibiting the production of inflammatory mediators.
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참고문헌 (22)
참고문헌 신청
Liu J / 2013 / Determination of the content of rosmarinic acid by HPLC and analytical comparison of volatile constituents by GC-MS in different parts of Perilla frutescens (L.) Britt / Chem Cent J 7:61
Hyun-Ah Lee / 2012 / Anti-inflammatory Effect of Perilla frutescens (L.) Britton var. frutescens Extract in LPS-stimulated RAW 264.7 Macrophages / Preventive Nutrition and Food Science 17(2):109~115
Meng L / 2008 / Antioxidant activities of polyphenols extracted from Perilla frutescens varieties / Molecules 14:133~140
Makino T / 2003 / Anti-allergic effect of Perilla frutescens and its active constituents / Phytother Res 17:240~243
Ueda H / 2003 / Inhibitory effect of Perilla leaf extract and luteolin on mouse skin tumor promotion / Biol Pharm Bull 26:560~563
Hyun-Ah Lee / 2012 / Anti-inflammatory Effect of Perilla frutescens (L.) Britton var. frutescens Extract in LPS-stimulated RAW 264.7 Macrophages / Preventive Nutrition and Food Science 17(2):109~115
Meng L / 2008 / Antioxidant activities of polyphenols extracted from Perilla frutescens varieties / Molecules 14:133~140
Makino T / 2003 / Anti-allergic effect of Perilla frutescens and its active constituents / Phytother Res 17:240~243
Ueda H / 2003 / Inhibitory effect of Perilla leaf extract and luteolin on mouse skin tumor promotion / Biol Pharm Bull 26:560~563
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