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자료유형
학술저널
저자정보
Shaikh J. Mohiuddin (Department of Biotechnology Bangabandhu Sheikh Mujubur Rahman Agricultural University Bangladesh) Md. Ashraful Haque (Department of Biotechnology Bangabandhu Sheikh Mujubur Rahman Agricultural University Bangladesh) Md. Manjurul Haque (Department of Environmental Science Bangabandhu Sheikh Mujubur Rahman Agricultural University Bang) Md. Tofazzal Islam (Department of Biotechnology Bangabandhu Sheikh Mujubur Rahman Agricultural University Bangladesh) Partha S Biswas (plant Breeding Division Bangladesh Rice Research Institute Bangladesh)
저널정보
한국육종학회 Plant Breeding and Biotechnology Plant Breeding and Biotechnology Vol.8 No.4
발행연도
2020.1
수록면
327 - 340 (14page)

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Molecular mapping and application of quantitative trait loci (QTL) associated with a higher level of grain Zinc is aviable option to enhance zinc content in rice through breeding. An F2 population derived from a cross between a high yielding variety,BRRI dhan28, and a locally adapted Zn enriched cultivar, Kalobokri was used to map QTLs associated with higher levels of Zn in ricegrain. The F2:3 progenies varied significantly (P < 0.0001) in Zinc contents with a mean value remarkably higher than those in thesuperior parent. Through marker by trait analysis using IciMapping, we detected a large-effect QTL, qGZn3 on chromosome 3 betweenRM5419 and RM1164 spanning 1.83 Mb interval at the 0.05 level of significance with a threshold LOD of 10.61. This QTL showed a21.1% (R2value) contribution to the total phenotypic variation for zinc content in the unpolished rice grains with 4.68 μg/g additiveeffect of Kalobokri alleles. We also detected 11 metal homeostasis related genes within the interval of qGZn3. In-silico analysis showedthat four expressed sequence tags of one candidate gene (LOC_Os03g22810) encoding Cu/Zn superoxide dismutase, a metal-bindingprotein, are highly active in the endosperm as well as in the embryonic tissue of rice grain. Taken together, our results suggest thatqGZn3 is a major QTL associated with high grain Zn content in the F2 progenies of rice. Our findings offer valuable genetic resourcesto facilitate breeding for high yielding and Zinc-enriched rice variety.

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