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논문 기본 정보

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학술저널
저자정보
Hélène Sénéchal (Armand Trousseau Children Hospital) Sanaz Keykhosravi (Armand Trousseau Children Hospital) Rémy Couderc (Armand Trousseau Children Hospital) Marie-Ange Selva (Armand Trousseau Children Hospital) Youcef Shahali (Razi Vaccine and Serum Research Institute) Tomoyasu Aizawa (Hokkaïdo University) Jean-Marc Busnel (Life Sciences Research) Rihane Arif (Life Sciences Research) Inna Mercier (Pasteur Institute Medical Center) Nhan Pham-Thi (Pasteur Institute Medical Center) Denis-André Charpin (Aix-Marseille University) Pascal Poncet (Armand Trousseau Children Hospital)
저널정보
대한천식알레르기학회(구 대한알레르기학회) Allergy, Asthma & Immunology Research Allergy, Asthma & Immunology Research Vol.11 No.1
발행연도
2019.1
수록면
143 - 151 (9page)

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A pollen/food-associated syndrome (PFAS) has been described between peach and cypress pollen. Cross-reactive allergens were characterized which belong to the Gibberellin-regulated protein (GRP) family, BP14 in cypress pollen and Pru p 7 in peach. GRP are small cationic protein with anti-microbial properties. A patient suffering from a peach/cypress syndrome was explored clinically and biologically using 2 types of immunoglobulin E (IgE) multiarray microchip, immunoblots and a basophil activation test to assess the clinical relevance of various extracts and purified allergens from fruits or cypress pollen. In addition to PR10 sensitization, the patient showed specific IgE to Pru p 7, BP14 and allergen from pomegranate. These last 3 allergens and allergenic sources are able to induce ex vivo basophil activation characterized by the monitoring of the expression of CD63 and CD203c, both cell surface markers correlated with a basophil mediator release. Up to 100% of cells expressed CD203c at 50 ng/mL of BP14 protein. In contrast, snakin-1, a GRP from potato sharing 82% sequence identity with Pru p 7 did not activate patient's basophils. These results strongly suggest that, like Pru p 7, BP14 is a clinically relevant allergenic GRP from pollen. Allergen members of this newly described protein family are good candidates for PFAS where no cross-reactive allergens have been characterized.

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