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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 35 - 43 (9page)
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초록· 키워드
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BackgroundFetal bovine serum (FBS) has been used to support the growth and proliferation of mam-malian cells for decades. Owing to several risk factors associated with FBS, several trials have been conducted to evaluate substitutes to FBS with the same efficiency and the lower risk issues.MethodsIn this study, human platelet lysate (HPL) derived from activated human platelets was evaluated as an alternative to FBS due to the associated risk factors. To evaluate the effi-ciency of the preparation process, platelet count was performed before and after activation. The concentrations of several growth factors and proteins were measured to investigate HPL efficiency. HPL stability was studied at regular intervals, and optimal hep-arin concentration required to prevent gel formation in various media was determined. The biological activity of HPL and FBS was compared by evaluating the growth perform-ance of Vero and Hep-2 cell lines.ResultsResult of platelet count assay revealed the efficiency of HPL preparation process. Growth factor concentrations in HPL were significantly higher than those in FBS, while the protein content of HPL was lower than that of FBS. Stability study data showed that the prepared HPL was stable for up to 15 months at -20oC. Ideal heparin concentration to be used in different media was dependent on calcium concentration. Results of cell viability assay showed that HPL was superior to FBS in supporting the growth and proliferation of Vero and Hep-2 cells. ConclusionThe HPL prepared by the mechanical activation of platelets may serve as an efficient alter-native to FBS in cell culture process.
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참고문헌 (29)
참고문헌 신청
Butler M / 2003 / Animal cell culture and technology / Taylor & Francis
Jochems CE / 2002 / The use of fetal bovine serum: ethical or scientific problem? / Altern Lab Anim 30:219~227
Montagnon BJ / 1989 / Polio and rabies vaccines produced in continuous cell lines : a reality for Vero cell line / Dev Biol Stand 70:27~47
Barrett PN / 2009 / Vero cell platform in vaccine production : moving towards cell culture-based viral vaccines / Expert Rev Vaccines 8:607~618
Kenny MT / 1975 / Microassay of measles and mumps virus and antibody in VERO cells / J Biol Stand 3:291~306
Jochems CE / 2002 / The use of fetal bovine serum: ethical or scientific problem? / Altern Lab Anim 30:219~227
Montagnon BJ / 1989 / Polio and rabies vaccines produced in continuous cell lines : a reality for Vero cell line / Dev Biol Stand 70:27~47
Barrett PN / 2009 / Vero cell platform in vaccine production : moving towards cell culture-based viral vaccines / Expert Rev Vaccines 8:607~618
Kenny MT / 1975 / Microassay of measles and mumps virus and antibody in VERO cells / J Biol Stand 3:291~306
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