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논문 기본 정보

자료유형
학술저널
저자정보
조혜란 (인천대학교) Aeri Lee (인천대학교) 김교범 (인천대학교)
저널정보
한국생체재료학회 생체재료학회지 생체재료학회지 제22권 제1호
발행연도
2018.3
수록면
28 - 37 (10page)
DOI
10.1186/s40824-018-0116-z

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Background: Fetal bovine serum (FBS) is the most essential supplement in culture media for cellular proliferation, metabolism, and differentiation. However, due to a limited supply and subsequently rising prices, a series of studies have investigated a biological feasibility of replaceable serums to substitute FBS. Along with the increasing interests to manufacture stem cell-based cellular products, optimizing the composition of culture media including serums and exogenous growth factors (GFs) is of importance. In this experiment, the effect of bovine serum (BS) and newborn calf serum (NCS) on proliferation and chondrogenic differentiation capacity of human adipose derived stem cells (ADSCs) was evaluated, especially in the chondrogenically supplemented culture condition. Methods: ADSCs were chondrogenically cultured with FBS, BS, and NCS for 14 days. For the acceleration of in vitro chondrogenesis, exogenous insulin-like growth factor and transforming growth factor-β3 were added. Viability and proliferation of ADSCs were evaluated using Live/Dead fluorescence staining and DNA amount, respectively. To investigate a chondrogenic differentiation, a series of assays were performed including a quantification of glycosaminoglycan deposition, alcian blue staining, and RT-PCR analysis for type II collagen, aggrecan and Sox-9 genes. Results: The results demonstrated that proliferation of ADSCs was facilitated in FBS condition as compared with other serum types. For chondrogenic marker gene expression, serum substitutes enhanced Sox-9 expression level on day 14. The deposition of glycosaminoglycan was more facilitated in BS condition regardless of additional chondrogenic GFs. Conclusion: It could be presumably speculated that serum types and exogenous supplements of GFs could also be important parameters to optimize culture media composition, especially in order to maintain the enhanced levels of both proliferation and chondrogenic differentiation of ADSCs during expansion.
#Fetal bovine serum #Adipose derived stem cell #Chondrogenic differentiation #Serum substitutes

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참고문헌 (49)

참고문헌 신청
Lai JH / 2015 / Interaction between osteoarthritic chondrocytes and adipose-derived stem cells is dependent on cell distribution in three-dimension and transforming growth factor-beta3 induction / Tissue Eng A 21(5-6):992~1002 Crossref Waters HA / 2013 / Co-culture of adipose derived stem cells and chondrocytes with surface modifying proteins induces enhanced cartilage tissue formation / Journal of investigative surgery : the official journal of the Academy of Surgical Research 26(3):118~126 Crossref Jo CH / 2014 / Intra-articular injection of mesenchymal stem cells for the treatment of osteoarthritis of the knee: a proof-of-concept clinical trial / Stem Cells 32(5):1254~1266 Crossref Kim YS / 2016 / Injection of mesenchymal stem cells as a supplementary strategy of marrow stimulation improves cartilage regeneration after lateral sliding calcaneal osteotomy for Varus ankle osteoarthritis: clinical and second-look arthroscopic results / Arthroscopy 32(5):878~889 Crossref Kim YS / 2015 / Mesenchymal stem cell implantation in osteoarthritic knees: is fibrin glue effective as a scaffold? / Am J Sports Med 43(1):176~185 Crossref

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