토끼의 이개와 비중격에서 채취한 연골모세포의 단층배양 조건에 대한 영향인자

진경석; 박소영; 김병국; 최하나; 정영훈; 오정훈

추천

검색

자료유형: 학술저널

저자정보: 진경석 박소영 김병국 최하나 정영훈 오정훈

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제58권 제2호

발행연도: 2015.1

수록면: 95 - 100 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background and Objectives In tissue engineering of the cartilage, cellular expansion is an essential step for creating useful amounts of tissue required. Many variables such as media composition, growth factors and cell seeding density have been utilized to stimulate the ability of cellular proliferation and to reduce the number of expansion in monolayer. The object of this study was to evaluate the effects of human serum (HS) and rabbit serum (RS) on cellular proliferation of auricular and nasal septal chondrocytes of the rabbit. Materials and Method Auricular and nasal septal chondrocytes of the New Zealand White Rabbit were expanded in monolayer in medium supplemented with 2% RS, 5% RS, 10% RS, or 10% HS with or without adding of growth factors. The effect of the supplements on the cellular proliferation was compared. Results The proliferation yield of chondrocytes grown in RS was comparable to that with HS. Both serums results in increased proliferation; however, the amount of cellular proliferation was not correlated with the concentration of the serum. The proliferation rate of nasal septal chondrocytes was higher than that of auricular chondrocytes. Conclusion Both HS and RS resulted in the increased proliferation of rabbit auricular and nasal septal chondrocytes. The results show that the determination of appropriate types and concentrations of serum is required for cell expansion in constructing tissue-engineered cartilage.

#Auricle #Chondrocyte #Nasal septum #Proliferation #Serum

참고문헌 (20)

참고문헌 신청

Alexander TH / 2006 / Human serum for tissue engineering of human nasal septal cartilage / Otolaryngol Head Neck Surg 135 (3) : 397 ~ 403

Alexander TH / 2010 / Insulin-like growth factor-I and growth differentiation factor-5 promote the formation of tissue-engineered human nasal septal cartilage / Tissue Eng P

Bujia J / 1994 / Effect of growth factors on cell proliferation by human nasal septal chondrocytes cultured in monolayer / Acta Otolaryngol 114 (5) : 539 ~ 543

Chia SH / 2004 / Tissue-engineered human nasal septal cartilage using the alginaterecovered-chondrocyte method / Laryngoscope 114 (1) : 38 ~ 45

Chung C / 2006 / Effects of auricular chondrocyte expansion on neocartilage formation in photocrosslinked hyaluronic acid networks / Tissue Eng 12 (9) : 2665 ~ 2673

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!