지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Osteoarthritis is a disorder characterized by a loss of cartilage as common aging-associated disease in humans and animals. However, unlike human clinical trials, investigational studies in pet animals are constrained by a lack of interest and funds. In addition, pet owners would often prefer the lowest cost method to treat arthritis of pet animals. Here, we report the outstanding and inexpensive way to prepare chondrocytes for cartilage repair using rabbit adipose derived mesenchymal stem cells (MSCs). This study focused on the development and enhancement of pre-chondrogenic condensation under external electric fields even without additional growth factors. We found that highly compact structures were formed within 3 days in micromass cultures of rabbit MSCs under electrical stimulation (ES), showing increased COL2A1 gene expression compared with their control 3D micromass cultures and 2D monolayer cultures. We further found that ES enhanced the production of proteoglycan, a highly produced extracellular matrix component in chondrocytes. Collectively, these results provide the commercial potential of electrical stimulation driving chondrogenesis of mesenchymal stem cells for repair of cartilage, which is a budget-friendly regimen.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Radisic M / 2004 / Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds / Proc. Natl Acad. Sci. USA 101(52):18129~18134
Titushkin I / 2009 / Regulation of cell cytoskeleton and membrane mechanics by electric field: role of linker proteins / Biophys. J. 96(2):717~728
Sheikh AQ / 2013 / Regulation of endothelial MAPK/ERK signalling and capillary morphogenesis by low-amplitude electric field / J. R. Soc. Interface 10(78):20120548
Zhang J / 2014 / A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling / PLoS ONE 9(3):e92252
Rackauskas G / 2014 / Subthreshold High-Frequency Electrical Field Stimulation Induces VEGF Expression in Cardiomyocytes / Cell Transplant 24(8):1653~1659
Titushkin I / 2009 / Regulation of cell cytoskeleton and membrane mechanics by electric field: role of linker proteins / Biophys. J. 96(2):717~728
Sheikh AQ / 2013 / Regulation of endothelial MAPK/ERK signalling and capillary morphogenesis by low-amplitude electric field / J. R. Soc. Interface 10(78):20120548
Zhang J / 2014 / A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling / PLoS ONE 9(3):e92252
Rackauskas G / 2014 / Subthreshold High-Frequency Electrical Field Stimulation Induces VEGF Expression in Cardiomyocytes / Cell Transplant 24(8):1653~1659
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Exogenous electrical stimulation drives differentiation of bone marrow derived mesenchymal stem cells toward chondrocytes
예방수의학회지
2019 .01
-
Differentiation potential of mesenchymal stem cells through electrical stimulation
예방수의학회지
2019 .01
-
Current perspectives in stem cell therapies for osteoarthritis of the knee
Journal of Yeungnam Medical Science
2020 .01
-
Effect of alginate concentration on chondrogenesis of co-cultured human adipose-derived stem cells and nasal chondrocytes: a biological study
생체재료학회지
2017 .12
-
High-glutathione mesenchymal stem cells isolated using the FreSHtracer probe enhance cartilage regeneration in a rabbit chondral defect model
생체재료학회지
2023 .03
-
Cryopreservation of Cartilage for Transplantation in Rhinoplasty: A Literature Review
대한이비인후-두경부외과학회지
2016 .01
-
Advanced Research on Stem Cell Therapy for Hepatic Diseases: Potential Implications of a Placenta-derived Mesenchymal Stem Cell-based Strategy
Hanyang Medical Reviews
2015 .01
-
토끼의 이개와 비중격에서 채취한 연골모세포의 단층배양 조건에 대한 영향인자
대한이비인후-두경부외과학회지
2015 .01
-
Regulation of Cartilage Development and Diseases by Transcription Factors
대한골대사학회지
2017 .08
-
Differentiation of Human Mesenchymal Stem Cells towards Neuronal Lineage: Clinical Trials in Nervous System Disorders
Biomolecules & Therapeutics(구 응용약물학회지)
2020 .01
-
Roles of Mesenchymal Stem Cells in Tissue Regeneration and Immunomodulation
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Comparative characteristic study from bone marrow-derived mesenchymal stem cells
Journal of Veterinary Science
2021 .11
-
줄기세포를 이용한 골관절염 치료
대한내과학회지
2019 .04
-
Progress and emerging techniques for biomaterial-based derivation of mesenchymal stem cells (MSCs) from pluripotent stem cells (PSCs)
생체재료학회지
2023 .03
-
골관절염에서 줄기세포를 이용한 연골 재생의 최신 지견
대한정형외과학회지
2019 .12
-
Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering
생체재료학회지
2019 .01
-
Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
Journal of Veterinary Science
2017 .06
-
하이드로젤 지지체 기반 3차원 환경에서 개 간엽줄기세포의 분화능 분석
Korean Journal of Veterinary Research(대한수의학회지)
2019 .02
-
Oxysterol 25-hydroxycholesterol as a metabolic pathophysiological factors of osteoarthritis induces apoptosis in primary rat chondrocytes
The Korean Journal of Physiology & Pharmacology
2020 .01
-
Establishment of bovine tongue epithelium-derived mesenchymal stem cells
예방수의학회지
2017 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0