지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Electrical stimulation (ES) is known to guide the development and regeneration of many tissues. Use of low-frequency ES for therapeutic purposes has been increasing during the last decades. Mesenchymal stem cells (MSCs) represent an appealing alternative cell source for cartilage repair. There are studies that induce differentiation into cartilage cells by treating the growth factors in stem cells or altering the properties of stem cells by genetic modification. In this study, we exposed equine adipose tissue-derived MSCs (eAD-MSCs) to ES and assessed changes in the chondrogenic differentiation potential. The cells obtained from equine adipose tissue attached to culture plates and expanded in vitro. Flow cytometric analysis at third passage indicated that the cells were strongly positive for CD44, CD90, and CD105, but negative for CD13, CD34, and CD45. Next, ES was applied to eAD-MSCs cultured under condition of high-density micromass under ES of 10 V/cm, with duration of 10 ms and a frequency of 2.0 Hz for three days. Gene expression of chondrogenic markers such as collagen type II, Aggrecan, and Sox9 was analyzed at three days of ES. As a result, we observed the differentiation potential of eAD-MSCs into chondrocytes by specific ES in absence of exogenous growth factors. We also found that ES upregulated the expression of heat shock protein 70, which affects cartilage formation. This study may contribute to the differentiation of MSCs into chondrogenic lineage under specific ES condition.
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참고문헌 (27)
참고문헌 신청
Becker AJ / 1963 / Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells / Nature 197:452~454
Carrade DD / 2012 / Comparative analysis of the immunomodulatory properties of equine adult-derived mesenchymal stem cells / Cell Med 4:1~11
Chan YC / 2013 / Electrical stimulation promotes maturation of cardiomyocytes derived from human embryonic stem cells / J Cardiovasc Transl Res 6:989~999
Chen J / 2014 / Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culture / PLoS One 9:e91561
Daugaar M / 2007 / The heat shock protein 70 family: highly homologous proteins with overlapping and distinct functions / FEBS Lett 581:3702~3710
Carrade DD / 2012 / Comparative analysis of the immunomodulatory properties of equine adult-derived mesenchymal stem cells / Cell Med 4:1~11
Chan YC / 2013 / Electrical stimulation promotes maturation of cardiomyocytes derived from human embryonic stem cells / J Cardiovasc Transl Res 6:989~999
Chen J / 2014 / Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culture / PLoS One 9:e91561
Daugaar M / 2007 / The heat shock protein 70 family: highly homologous proteins with overlapping and distinct functions / FEBS Lett 581:3702~3710
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