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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1,031 - 1,039 (9page)
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Purpose: Most studies on immune tolerance of mesenchymal stem cells (MSCs) have been performed using MSCs derived from bone marrow, cord blood, or adipose tissue. MSCs also exist in the craniofacial area, specifically in teeth. The purpose of this study was to evaluate the mechanisms of immune tolerance of dental pulp-derived MSC (DP-MSC) in vitro and in vivo.
Materials and Methods: We isolated DP-MSCs from human dental pulp and co-cultured them with CD4+ T-cells. To evaluate the role of cytokines, we blocked TGF-β and IL-10, separately and together, in co-cultured DP-MSCs and CD4+ T-cells. We analyzed CD25 and FoxP3 to identify regulatory T-cells (Tregs) by fluorescence-activated cell sorting (FACS) and real-time PCR. We performedalloskin grafts with and without DP-MSC injection in mice. We performed mixed lymphocyte reactions (MLRs) to check immune tolerance.
Results: Co-culture of CD4+ T-cells with DP-MSCs increased the number of CD4+CD25+FoxP3+ Tregs (p<0.01). TGF-β or/and IL-10 blocking suppressed Treg induction in co-cultured cells (p<0.05). TGF-β1 mRNA levels were higher in co-cultured DP-MSCs and in co-cultured CD4+ T-cells than in the respective monocultured cells. However, IL-10 mRNA levels were not different. There was no difference in alloskin graft survival rate and area between the DP-MSC injection group and the non-injection group. Nonetheless,MLR was reduced in the DP-MSC injected group (p<0.05).
Conclusion: DP-MSCs can modulate immune tolerance by increasing CD4+CD25+FoxP3+ Tregs. TGF-β1 and IL-10 are factors in the immune-tolerance mechanism. Pure DP-MSC therapy may not be an effective treatment for rejection, although it may module immune tolerance in vivo.
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참고문헌 (24)
참고문헌 신청
Di Ianni M / 2008 / Mesenchymal cells recruit and regulate T regulatory cells / Exp Hematol 36 : 309 ~ 318
Ding G / 2010 / Suppression of T cell proliferation by root apical papilla stem cells in vitro / Cells Tissues Organs 191 : 357 ~ 364
English K / 2009 / Cell contact, prostaglandin E(2) and transforming growth factor beta 1 play non-redundant roles in human mesenchymal stem cell induction of CD4+CD25(High)
Gronthos S / 2000 / Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo / Proc Natl Acad Sci U S A 97 : 13625 ~ 13630
Gronthos S / 2002 / Stem cell properties of human dental pulp stem cells / J Dent Res 81 : 531 ~ 535
Ding G / 2010 / Suppression of T cell proliferation by root apical papilla stem cells in vitro / Cells Tissues Organs 191 : 357 ~ 364
English K / 2009 / Cell contact, prostaglandin E(2) and transforming growth factor beta 1 play non-redundant roles in human mesenchymal stem cell induction of CD4+CD25(High)
Gronthos S / 2000 / Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo / Proc Natl Acad Sci U S A 97 : 13625 ~ 13630
Gronthos S / 2002 / Stem cell properties of human dental pulp stem cells / J Dent Res 81 : 531 ~ 535
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