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논문 기본 정보

자료유형
학술저널
저자정보
Wataru Katagiri (Niigata University) Takamasa Kawai (Niigata University) Masashi Osugi (Niigata University) Yukiko Sugimura-Wakayama (Niigata University) Kohei Sakaguchi (Niigata University) Taku Kojima (Niigata University) Tadaharu Kobayashi (Niigata University)
저널정보
대한악안면성형재건외과학회 Maxillofacial Plastic Reconstructive Surgery Maxillofacial Plastic Reconstructive Surgery 제39권 제3호
발행연도
2017.3
수록면
1 - 8 (8page)
DOI
10.1186/s40902-017-0106-4

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AbstractBackground: For an effective bone graft for reconstruction of the maxillofacial region, an adequate vascularnetwork will be required to supply blood, osteoprogenitor cells, and growth factors. We previously reported thatthe secretomes of bone marrow-derived mesenchymal stem cells (MSC-CM) contain numerous growth factors suchas insulin-like growth factor (IGF)-1, transforming growth factor (TGF)-β1, and vascular endothelial growth factor(VEGF), which can affect the cellular characteristics and behavior of regenerating bone cells. We hypothesized thatangiogenesis is an important step for bone regeneration, and VEGF is one of the crucial factors in MSC-CM thatwould enhance its osteogenic potential. In the present study, we focused on VEGF in MSC-CM and evaluated theangiogenic and osteogenic potentials of MSC-CM for bone regeneration. Methods: Cytokines in MSC-CM were measured by enzyme-linked immunosorbent assay (ELISA). Human umbilicalvein endothelial cells (HUVECs) were cultured with MSC-CM or MSC-CM with anti-VEGF antibody (MSC-CM + anti-VEGF)for neutralization, and tube formation was evaluated. For the evaluation of bone and blood vessel formation withmicro-computed tomography (micro-CT) and for the histological and immunohistochemical analyses, a rat calvarialbone defect model was used. Results: The concentrations of IGF-1, VEGF, and TGF-β1 in MSC-CM were 1515.6 ± 211.8 pg/mL, 465.8 ± 108.8 pg/mL, and339.8 ± 14.4 pg/mL, respectively. Tube formation of HUVECs, bone formation, and blood vessel formation were increasedin the MSC-CM group but decreased in the MSC-CM + anti-VEGF group. Histological findings suggested that new boneformation in the entire defect was observed in theMSC-CMgroup although it was decreased in the MSC-CM + anti-VEGFgroup. Immunohistochemistry indicated that angiogenesis and migration of endogenous stem cells were much moreabundant in the MSC-CM group than in the MSC-CM + anti-VEGF group. Conclusions: VEGF is considered a crucial factor in MSC-CM, and MSC-CM is proposed to be an adequate therapeuticagent for bone regeneration with angiogenesis
#Secretome #Conditioned medium #Cytokine #Mesenchymal stem cells #Angiogenesis #Bone

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참고문헌 (28)

참고문헌 신청
Aghaloo TL / 2007 / Which hard tissue augmentation techniques are the most successful in furnishing bony support for implant placement? / Int J Oral Maxillofac Implants 22(suppl):49~70 google schola Langer R / 1993 / Tissue engineering / Science 260:920~926 google schola Yamada Y / 2004 / Autogenous injectable bone for regeneration with mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP)—tissue-engineered bone regeneration / Tissue Eng 10:955~964 google schola Yamada Y / 2013 / Injectable bonetissue engineeringusingexpanded mesenchymal stem cells / Stem Cells 31:572~580 google schola Osugi M / 2012 / Conditioned media from mesenchymal stem cells enhancedbone regeneration in rat calvarial bone defects / Tissue Eng Part A 18:14779~11489 google schola

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