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논문 기본 정보

자료유형
학술저널
저자정보
Hyun Cheol Bae (Seoul National University Hospital) Hee Jung Park (Seoul National University Hospital) Sun Young Wang (Seoul National University Hospital) Ha Ru Yang (Seoul National University Hospital) Myung Chul Lee (Seoul National University Hospital) 한혁수 (서울대학교병원)
저널정보
한국생체재료학회 생체재료학회지 생체재료학회지 제22권 제4호
발행연도
2018.12
수록면
271 - 278 (8page)
DOI
https://doi.org/10.1186/s40824-018-0134-x

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Background: The chondrogenic differentiation of mesenchymal stem cells (MSCs) is regulated by many factors, including oxygen tensions, growth factors, and cytokines. Evidences have suggested that low oxygen tension seems to be an important regulatory factor in the proliferation and chondrogenic differentiation in various MSCs. Recent studies report that synovium-derived mesenchymal stem cells (SDSCs) are a potential source of stem cells for the repair of articular cartilage defects. But, the effect of low oxygen tension on the proliferation and chondrogenic differentiation in SDSCs has not characterized. In this study, we investigated the effects of hypoxia on proliferation and chondrogenesis in SDSCs. Method: SDSCs were isolated from patients with osteoarthritis at total knee replacement. To determine the effect of oxygen tension on proliferation and colony-forming characteristics of SDSCs, A colony-forming unit (CFU) assay and cell counting-based proliferation assay were performed under normoxic (21% oxygen) or hypoxic (5% oxygen). For in vitro chondrogenic differentiation, SDSCs were concentrated to form pellets and subjected to conditions appropriate for chondrogenic differentiation under normoxia and hypoxia, followed by the analysis for the expression of genes and proteins of chondrogenesis. qRT-PCR, histological assay, and glycosoaminoglycan assays were determined to assess chondrogenesis. Results: Low oxygen condition significantly increased proliferation and colony-forming characteristics of SDSCs compared to that of SDSCs under normoxic culture. Similar pellet size and weight were found for chondrogensis period under hypoxia and normoxia condition. The mRNA expression of types II collagen, aggrecan, and the transcription factor SOX9 was increased under hypoxia condition. Histological sections stained with Safranin-O demonstrated that hypoxic conditions had increased proteoglycan synthesis. Immunohistochemistry for types II collagen demonstrated that hypoxic culture of SDSCs increased type II collagen expression. In addition, GAG deposition was significantly higher in hypoxia compared with normoxia at 21 days of differentiation. Conclusion: These findings show that hypoxia condition has an important role in regulating the synthesis ECM matrix by SDSCs as they undergo chondrogenesis. This has important implications for cartilage tissue engineering applications of SDSCs.
#Synovium-derived mesenchymal stem cells (SDSCs) #Hypoxia #Chondrogensis #Oxygen

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참고문헌 (35)

참고문헌 신청
Pastides P / 2013 / Stem cell therapy for human cartilage defects : a systematic review / Osteoarthr Cartil 21:646~654 google schola Bornes TD / 2014 / Mesenchymal stem cells in the treatment of traumatic articular cartilage defects : a comprehensive review / Arthritis Res Ther 16:432 google schola Sakaguchi Y / 2005 / Comparison of human stem cells derived from variousmesenchymal tissues : superiority of synovium as a cell source / Arthritis Rheum 52:2521 google schola Kubosch EJ / 2018 / The potential for synovium-derived stem cells in cartilage repair / Curr Stem Cell Res Ther 13:174~184 google schola Yusuke O / 2015 / Purified human synovium mesenchymal stem cells as a good resource for cartilage regeneration / PLoS One 10:e129096 google schola

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