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- 대한천식알레르기학회(구 대한알레르기학회) Allergy, Asthma & Immunology Research Allergy, Asthma & Immunology Research Vol.7 No.3
- 발행연도
- 2015.1
- 수록면
- 283 - 289 (7page)
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Purpose: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease. Methods: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA. Results: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract. Conclusions: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.
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참고문헌 (26)
참고문헌 신청
Matsumura Y. / 2012 / Role of allergen source-derived proteases in sensitization via airway epithelial cells / J Allergy (Cairo) 2012:903659
Bhat RK / 2003 / German cockroach extract increases bronchial epithelial cell interleukin-8 expression / Clin Exp Allergy 33:35~42
Jeong KY / 2013 / Identification of novel allergenic components from German cockroach fecal extract by a proteomic approach / Int Arch Allergy Immunol 161:315~324
Robinson BW / 1990 / Allergens as proteases: an Aspergillus fumigatus proteinase directly induces human epithelial cell detachment / J Allergy Clin Immunol 86:726~731
Stewart GA / 1991 / Faecally derived hydrolytic enzymes from Dermatophagoides pteronyssinus: physicochemical characterisation of potential allergens / Int Arch Allergy Appl Immunol 95:248~256
Bhat RK / 2003 / German cockroach extract increases bronchial epithelial cell interleukin-8 expression / Clin Exp Allergy 33:35~42
Jeong KY / 2013 / Identification of novel allergenic components from German cockroach fecal extract by a proteomic approach / Int Arch Allergy Immunol 161:315~324
Robinson BW / 1990 / Allergens as proteases: an Aspergillus fumigatus proteinase directly induces human epithelial cell detachment / J Allergy Clin Immunol 86:726~731
Stewart GA / 1991 / Faecally derived hydrolytic enzymes from Dermatophagoides pteronyssinus: physicochemical characterisation of potential allergens / Int Arch Allergy Appl Immunol 95:248~256
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