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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Persptectives Vol.6 No.6
- 발행연도
- 2015.1
- 수록면
- 357 - 362 (6page)
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초록· 키워드
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Objectives: The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)- 7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGF-R2)]. Methods: We assessed the concentration changes of these proteins in 30 plasma and serum samples subjected to three, four, or five freezee-thaw cycles, and compared these with the concentration changes in the samples that were subjected to two freezee-thaw cycles before analysis. Results: Repeated freezing and thawing by up to five cycles did not modify the plasma and serum concentrations of interferon-γ, IL-8, and VEGF-R2, while levels of MMP-7, tumor necrosis factor-α, and VEGF were significantly changed in both plasma and serum samples. Moreover, MMP-7 and VEGF concentrations tended to increase with freezee-thaw cycles. They were more elevated in plasma samples (up to about 15%) than in serum samples (up to about 7%), suggesting that serum is the preferred sample type for the analysis of circulating proteins. Conclusion: This is the first report on the effect of repeated freezing and thawing on plasma concentrations of MMP-7 and VEGF-R2. Our findings propose that researchers should consider the number of freezee-thaw cycles to select plasma or serum samples, depending on the type of analyte.
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참고문헌 (24)
참고문헌 신청
Anderson NL / 2002 / The human plasma proteome: history, character, and diagnostic prospects / Mol Cell Proteomics 1(11):845~867
Pieper R / 2003 / The human serum proteome:display of nearly 3700 chromatographically separated protein spots on two-dimensional electrophoresis gels and identification of 325 distinct proteins / Proteomics 3(7):1345~1364
Kaya H / 2015 / Fibulin-3 as a diagnostic biomarker in patients with malignant mesothelioma / Asian Pac J Cancer Prev 16(4):1403~1407
Fang Z / 2014 / Clinical significance of stanniocalcin expression in tissue and serum of gastric cancer patients / Chin J Cancer Res 26(5):602~610
Moon HS / 2000 / Concentration of vascular endothelial growth factor (VEGF) and transforming growth factor-beta1(TGF-beta1) in the serum of patients with cervical cancer: prediction of response / Int J Gynecol Cancer 10(2):151~156
Pieper R / 2003 / The human serum proteome:display of nearly 3700 chromatographically separated protein spots on two-dimensional electrophoresis gels and identification of 325 distinct proteins / Proteomics 3(7):1345~1364
Kaya H / 2015 / Fibulin-3 as a diagnostic biomarker in patients with malignant mesothelioma / Asian Pac J Cancer Prev 16(4):1403~1407
Fang Z / 2014 / Clinical significance of stanniocalcin expression in tissue and serum of gastric cancer patients / Chin J Cancer Res 26(5):602~610
Moon HS / 2000 / Concentration of vascular endothelial growth factor (VEGF) and transforming growth factor-beta1(TGF-beta1) in the serum of patients with cervical cancer: prediction of response / Int J Gynecol Cancer 10(2):151~156
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