지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 279 - 282 (4page)
이용수
초록· 키워드
오류제보하기
In order to quantify compound K(CK), anticancer component of Panax ginseng C. A. Meyer, high titer rabbit polyclonal antibodies (pAbs) were raised against a conjugate of CK and bovine serum albumin coupled by a periodate oxidation method. Coating antigen (CK-OVA) was also prepared by the same method with OVA. As a result of optimization of antiserum dilution (2,000 fold), coating antigen (25 μg/ml) and other condition (incubation time, temperature and washing method), ELISA method for the determination of CK was established. The measuring range extended from 0.5 ng/ml to 25 ng/ml of CK. The antibodies exhibited minor or even no cross reactivities with protopanaxatriol (1.56%) and other tested ginsenosides, GRb₁ (0.11%), GRg₁ (0.07%) except protopanaxadiol (87.2%) from the structural similarity. And the antibody showed good correlation (r=0.987) between the assay values obtained by this ELISA method and HPLC. Therefore, the ELISA method could be very useful tools for the determination of CK in biological fluids because of their high sensitivity and specificity.
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Yosioka, I. / 1972 / Soil bacterial hydrolysis leading to genuine aglycone. V. On ginsenosides Rb1, Rb2 and Rc of the ginseng root saponins / Chem. Pharm. Bull 20:2418~2421
Zhou, W. / 2006 / Crystal structure and bioactivity of ginsenoside compound K / J. Asian Nat. Prod. Res 8:519~527
Zhang, Z. / 2013 / Compound K, a ginsenoside metabolite, inhibits colon cancer growth via multiple pathways including p53-p21 interactions / Int. J. Mol. Sci 14:2980~2995
Joh, E. H. / 2011 / Ginsenoside Rb1 and its metabolite compound K inhibit IRAK-1 activation-the key step of inflammation / Biochem. Pharmacol 82:278~286
Chen, J. / 2014 / Ginsenoside metabolite compound K alleviates adjuvant-induced arthritis by suppressing T cell activation / Inflammation 37:1608~1615
Zhou, W. / 2006 / Crystal structure and bioactivity of ginsenoside compound K / J. Asian Nat. Prod. Res 8:519~527
Zhang, Z. / 2013 / Compound K, a ginsenoside metabolite, inhibits colon cancer growth via multiple pathways including p53-p21 interactions / Int. J. Mol. Sci 14:2980~2995
Joh, E. H. / 2011 / Ginsenoside Rb1 and its metabolite compound K inhibit IRAK-1 activation-the key step of inflammation / Biochem. Pharmacol 82:278~286
Chen, J. / 2014 / Ginsenoside metabolite compound K alleviates adjuvant-induced arthritis by suppressing T cell activation / Inflammation 37:1608~1615
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development and Evaluation of Indirect ELISA for Detection of Antibodies to Getah Virus in Horse Serum
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2016 .06
-
Comparison of novel recombinant nucleoprotein ELISA with commercial ELISA for Newcastle disease
예방수의학회지
2021 .01
-
Quantification of Fucoidan by monoclonal antibody based sandwich ELISA
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera
Journal of Veterinary Science
2020 .07
-
Novel Sensitive, Two-site ELISA for the Quantification of Der f 1 Using Monoclonal Antibodies
Allergy, Asthma & Immunology Research
2021 .01
-
Development of monoclonal antibody capture ELISA for the detection of antibodies against transmissible gastroenteritis virus
한국가축위생학회지
2019 .01
-
Comparison and implications of the method for determination of algal toxins: ELISA and LC-MS/MS
환경독성보건학회 심포지엄 및 학술대회
2022 .05
-
Serodiagnosis of Extraintestinal Amebiasis : Retrospective Evaluation of the Diagnostic Performance of the Bordier® ELISA Kit
Parasites, Hosts and Diseases
2018 .02
-
Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis
Clinical and Experimental Vaccine Research
2018 .01
-
Development of an Indirect ELISA Featuring Plates Coated with Column Chromatographically Purified Canine Adenovirus Type-1 Antigen
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .03
-
Cytokeratin 15 is an Effective Indicator for Progression and Malignancy of Esophageal Squamous Cell Carcinomas
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Creatine Kinase (CK)-MB-to-Total-CK Ratio: a Laboratory Indicator for Primary Cancer Screening
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea : A Four-Year Follow-up
Parasites, Hosts and Diseases
2017 .06
-
AKT1-targeted proapoptotic activity of compound K in human breast cancer cells
Journal of Ginseng Research
2019 .10
-
Defining the Normal Trends of Serum Creatine Kinase Levels Following Spinal Surgery
Asian Spine Journal
2019 .01
-
ELISA for Quantitative Determination of Hepatitis B Virus Surface Antigen
Immune Network
2017 .12
-
Significance of Serology by Multi-Antigen ELISA for Tissue Helminthiases in Korea
Journal of Korean Medical Science
2017 .01
-
Development of a HA1-specific enzyme-linked immunosorbent assay against pandemic influenza virus A H1N1
Clinical and Experimental Vaccine Research
2019 .01
-
Ginsenoside compound K ameliorates palmitate-induced atrophy in C2C12 myotubes via promyogenic effects and AMPK/autophagy-mediated suppression of endoplasmic reticulum stress
Journal of Ginseng Research
2022 .05
-
Development and Evaluation of an Immunoglobulin Y-Based ELISA for Measuring Prostate Specific Antigen in Human Serum
Annals of Laboratory Medicine
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-518-000069177