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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.10
- 수록면
- 692 - 698 (7page)
이용수
초록· 키워드
오류제보하기
Background: Breast cancer is a severe disease and the second leading cause of cancer death in women worldwide. To surmount this, various diagnosis and treatment options for breast cancer have been developed. One of the most effective strategies for cancer treatment is to induce apoptosis using naturally occurring compounds. Compound K (CK) is a ginseng saponin metabolite generated by human intestinal bacteria. CK has been studied for its cardioprotective, antiinflammatory, and liver-protective effects; however, the role of CK in breast cancer is not fully understood.
Methods: To investigate the anticancer effects of CK in SKBR3 and MDA-MB-231 cells, cell viability assays and flow cytometry analysis were used. In addition, the direct targets of CK anticancer activity were identified using immunoblotting analysis and overexpression experiments. Invasion, migration, and clonogenic assays were carried out to determine the effects of CK on cancer metastasis.
Results: CK-induced cell apoptosis in SKBR3 cells as determined through 3-(4-5-dimethylthiazol-2-yl)-2- 5-diphenyltetrazolium bromide assays, propidium iodide (PI) and annexin V staining, and morphological changes. CK increased the cleaved forms of caspase-7, caspase-8, and caspase-9, whereas the expression of Bcl-2 was reduced by CK. In assays probing the cell survival pathway, CK activated only AKT1 and not AKT2. Moreover, CK inhibited breast cancer cell invasion, migration, and colony formation. Through regulation of AKT1 activity, CK exerts anticancer effects by inducing apoptosis.
Conclusion: Our results suggest that CK could be used as a therapeutic compound for breast cancer.
Methods: To investigate the anticancer effects of CK in SKBR3 and MDA-MB-231 cells, cell viability assays and flow cytometry analysis were used. In addition, the direct targets of CK anticancer activity were identified using immunoblotting analysis and overexpression experiments. Invasion, migration, and clonogenic assays were carried out to determine the effects of CK on cancer metastasis.
Results: CK-induced cell apoptosis in SKBR3 cells as determined through 3-(4-5-dimethylthiazol-2-yl)-2- 5-diphenyltetrazolium bromide assays, propidium iodide (PI) and annexin V staining, and morphological changes. CK increased the cleaved forms of caspase-7, caspase-8, and caspase-9, whereas the expression of Bcl-2 was reduced by CK. In assays probing the cell survival pathway, CK activated only AKT1 and not AKT2. Moreover, CK inhibited breast cancer cell invasion, migration, and colony formation. Through regulation of AKT1 activity, CK exerts anticancer effects by inducing apoptosis.
Conclusion: Our results suggest that CK could be used as a therapeutic compound for breast cancer.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (46)
참고문헌 신청
Siegel RL / 2015 / Cancer statistics / CA Cancer J Clin 65:5~29
Torre LA / 2012 / Global cancer statistics / CA Cancer J Clin 65:87~108
Joy A / 2015 / Systemic treatment approaches in her2-negative advanced breast cancerdguidance on the guidelines / Curr Oncol 22:S29
Witton CJ / 2003 / Expression of the HER1-4 family of receptor tyrosine kinases in breast cancer / J Pathol 200:290~297
Schmitt CA / 2003 / Senescence, apoptosis and therapydcutting the lifelines of cancer / Nat Rev Cancer 3:286
Torre LA / 2012 / Global cancer statistics / CA Cancer J Clin 65:87~108
Joy A / 2015 / Systemic treatment approaches in her2-negative advanced breast cancerdguidance on the guidelines / Curr Oncol 22:S29
Witton CJ / 2003 / Expression of the HER1-4 family of receptor tyrosine kinases in breast cancer / J Pathol 200:290~297
Schmitt CA / 2003 / Senescence, apoptosis and therapydcutting the lifelines of cancer / Nat Rev Cancer 3:286
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UCI(KEPA) : I410-ECN-0101-2020-524-000504733