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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 154 - 160 (7page)
이용수
초록· 키워드
오류제보하기
Background: Compound K (CK) is an active metabolite of ginseng saponin, ginsenoside Rb1, that has been shown to have ameliorative properties in various diseases. However, its role in inflammation and the underlying mechanisms are poorly understood. In this report, the antiinflammatory role of CK was investigated in macrophage-like cells.
Methods: The CK-mediated antiinflammatory mechanism was explored in RAW264.7 and HEK293 cells that were activated by lipopolysaccharide (LPS) or exhibited overexpression of known activation proteins. The mRNA levels of inflammatory genes and the activation levels of target proteins were identified by quantitative and semiquantitative reverse transcription polymerase chain reaction and Western blot analysis.
Results: CK significantly inhibited the mRNA expression of inducible nitric oxide synthase and tumor necrosis factor-α and morphological changes in LPS-activated RAW264.7 cells under noncytotoxic concentrations. CK downregulated the phosphorylation of AKT1, but not AKT2, in LPS-activated RAW264.7 cells. Similarly, CK reduced the AKT1 overexpression-induced expression of aldehyde oxidase 1, inter-leukin- 1β, interferon-β, and tumor necrosis factor-α in a dose-dependent manner.
Conclusion: Our results suggest that CK plays an antiinflammatory role during macrophage-mediated inflammatory actions by specifically targeting the AKT1-mediated signaling pathway.
Methods: The CK-mediated antiinflammatory mechanism was explored in RAW264.7 and HEK293 cells that were activated by lipopolysaccharide (LPS) or exhibited overexpression of known activation proteins. The mRNA levels of inflammatory genes and the activation levels of target proteins were identified by quantitative and semiquantitative reverse transcription polymerase chain reaction and Western blot analysis.
Results: CK significantly inhibited the mRNA expression of inducible nitric oxide synthase and tumor necrosis factor-α and morphological changes in LPS-activated RAW264.7 cells under noncytotoxic concentrations. CK downregulated the phosphorylation of AKT1, but not AKT2, in LPS-activated RAW264.7 cells. Similarly, CK reduced the AKT1 overexpression-induced expression of aldehyde oxidase 1, inter-leukin- 1β, interferon-β, and tumor necrosis factor-α in a dose-dependent manner.
Conclusion: Our results suggest that CK plays an antiinflammatory role during macrophage-mediated inflammatory actions by specifically targeting the AKT1-mediated signaling pathway.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (48)
참고문헌 신청
Janeway Jr CA / 2002 / Innate immune recognition / Annu Rev Immunol 20:197~216
Yi YS / 2017 / Caspase-11 non-canonical inflammasome : a critical sensor of intracellular lipopolysaccharide in macrophage-mediated inflammatory responses / Immunology 152:207~217
이영수 / 2016 / Folate Receptor-Targeted Diagnostics and Therapeutics for Inflammatory Diseases / Immune Network 16(6):337~343
Yi YS / 2014 / Functional roles of Syk in macrophage-mediated inflammatory responses / Mediators Inflamm 2014:270302
Yang Y / 2014 / Functional roles of p38 mitogen-activated protein kinase in macrophage-mediated inflammatory responses / Mediators Inflamm 2014:352371
Yi YS / 2017 / Caspase-11 non-canonical inflammasome : a critical sensor of intracellular lipopolysaccharide in macrophage-mediated inflammatory responses / Immunology 152:207~217
이영수 / 2016 / Folate Receptor-Targeted Diagnostics and Therapeutics for Inflammatory Diseases / Immune Network 16(6):337~343
Yi YS / 2014 / Functional roles of Syk in macrophage-mediated inflammatory responses / Mediators Inflamm 2014:270302
Yang Y / 2014 / Functional roles of p38 mitogen-activated protein kinase in macrophage-mediated inflammatory responses / Mediators Inflamm 2014:352371
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UCI(KEPA) : I410-ECN-0101-2019-524-000425176