Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells

Guihua Zhao; Lixin Zhang; Lisha Dai; Haozhi Xu; Ting Xiao; Chao Xu; Ting Xiao; Jin Li; Hui Sun; Beibei Zhou; Kun Yin

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자료유형: 학술저널

저자정보: Guihua Zhao (Shandong First Medical University & Shandong Academy of Medical Sciences) Lixin Zhang (Shandong First Medical University & Shandong Academy of Medical Sciences) Lisha Dai (Shandong First Medical University & Shandong Academy of Medical Sciences) Haozhi Xu (Shandong First Medical University & Shandong Academy of Medical Sciences) Ting Xiao (Shandong First Medical University & Shandong Academy of Medical Sciences) Chao Xu (Shandong First Medical University & Shandong Academy of Medical Sciences) Ting Xiao (Shandong First Medical University & Shandong Academy of Medical Sciences) Jin Li (Shandong First Medical University & Shandong Academy of Medical Sciences) Hui Sun (Shandong First Medical University & Shandong Academy of Medical Sciences) Beibei Zhou (Jining Blood Center) Kun Yin (Shandong First Medical University & Shandong Academy of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.60 No.4

발행연도: 2022.8

수록면: 241 - 246 (6page)

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Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.

#Toxoplasma gondii #cat intestinal epithelial cell #development #oocyst excretion #Chinese 1 genotype Wh6 strain

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