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논문 기본 정보

자료유형
학술저널
저자정보
Kim Hunseong (School of Food Science and Biotechnology College of Agriculture and Life Sciences Kyungpook Nationa) Cao Thao Quyen (Coastal Agriculture Research Institute Kyungpook National University Daegu 41566 Republic of Korea) Yeo Chae-eun (Department of Integrative Biotechnology Kyungpook National University Daegu 41566 Republic of Korea) Shin Seung Ho (Department of Food and Nutrition Institute of Agriculture and Life Science Gyeongsang National Univ) Kim Hiyoung (Department of Biomedical Science and Engineering Konkuk University Seoul 05029 Republic of Korea) Hong Dong-Hyuck (School of Bio-Industrial Machinery Engineering Kyungpook National University Daegu 41566 Republic o) 한동엽 (경북대학교)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제8호
발행연도
2022.8
수록면
976 - 981 (6page)
DOI
10.4014/jmb.2206.06037

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Phenanthrenes are bioactive phenolic compounds found in genus Dioscorea, in which they are distributed more in peel than in flesh. Recent studies on phenanthrenes from Dioscorea sp. peels have revealed the potential for valuable biomaterials. Herein, an analytical method using highperformance liquid chromatography (HPLC) for quantitation of bioactive phenanthrenes was developed and validated. The calibration curves were obtained using the phenanthrenes (1-3) previously isolated from Dioscorea batatas concentrations in the range of 0.625-20.00 μg/ml with a satisfactory coefficient of determination (R2) of 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) values of the isolated phenanthrenes ranged from 0.78-0.89 and 2.38-2.71 μg/ml, respectively. The intraday and interday precision ranged from 0.25-7.58%. The recoveries of the isolated phenanthrenes were from 95 to 100% at concentrations of 1.25, 2.5, and 5.0 μg/ml. Additionally, phenanthrenes (1-3) were found in all investigated peel extracts. Hence, the developed method was encouraging for the quantitative analysis of phenanthrenes in genus Dioscorea.

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