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논문 기본 정보

자료유형
학술저널
저자정보
박강원 (국립축산과학원) Hyeon Yang (Animal Biotechnology DivisionNational Institute of Animal Science RDA) Min Gook Lee (Rural Development Administration) 옥선아 (국림축산과학원) 위하연 (국립축산과학원 동물바이오공학과) 이풍연 (농촌진흥청 국립축산과학원) In-Sul Hwang (Columbia University) 유재규 (농촌진흥청 국립축산과학원) Choon Keun Park (Kangwon National University) Bo Ram Lee (National Institute of Animal Science Rural Development Administration)
저널정보
한국축산학회(구 한국동물자원과학회) 한국축산학회지 한국축산학회지 제64권 제6호
발행연도
2022.11
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1,105 - 1,116 (12page)

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Recently, we reported the robust in vitro three-dimensional (3D) expansion of intestinal organoids derived from adult bovine (> 24 months) samples. The present study aimed to establish an in vitro 3D system for the cultivation of intestinal organoids derived from growing cattle (12 months old) for practical use as a potential alternative to in vivo systems for various purposes. However, very few studies on the functional characterization and 3D expansion of adult stem cells from livestock species compared to those from other species are available. In this study, intestinal crypts, including intestinal stem cells, from the small intestines (ileum and jejunum) of growing cattle were isolated and long-term 3D cultures were successfully established using a scaffold-based method. Furthermore, we generated an apical-out intestinal organoid derived from growing cattle. Interestingly, intestinal organoids derived from the ileum, but not the jejunum, could be expanded without losing the ability to recapitulate crypts, and these organoids specifically expressed several specific markers of intestinal stem cells and the intestinal epithelium. Furthermore, these organoids exhibited key functionality with regard to high permeability for compounds up to 4 kDa in size (e.g., fluorescein isothiocyanate [FITC]-dextran), indicating that apical-out intestinal organoids are better than other models. Collectively, these results indicate the establishment of growing cattle-derived intestinal organoids and subsequent generation of apical-out intestinal organoids. These organoids may be valuable tools and potential alternatives to in vivo systems for examining host-pathogen interactions involving epithelial cells, such as enteric virus infection and nutrient absorption, and may be used for various purposes.

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