지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.9
- 수록면
- 463 - 491 (29page)
- DOI
- https://doi.org/10.1186/s40824-022-00264-8
이용수
초록· 키워드
오류제보하기
Background: Developing biocompatible nerve conduits that accelerate peripheral nerve regeneration, lengthen ing and functional recovery remains a challenge. The combined application of nerve microtissues and platelet-rich plasma (PRP) provides abundant Schwann cells (SCs) and various natural growth factors and can compensate for the deficiency of SCs in the nerve bridge, as well as the limitations of applying a single type of growth factor. Multimodal ultrasound evaluation can provide additional information on the stiffness and microvascular flow perfusion of the tis sue. This study was designed to investigate the effectiveness of a novel tissue-engineered nerve graft composed of an autogenous vein, nerve microtissues and PRP in reconstructing a 12-mm tibial nerve defect and to explore the value of multimodal ultrasound techniques in evaluating the prognosis of nerve repair. Methods: In vitro, nerve microtissue activity was first investigated, and the effects on SC proliferation, migration, fac tor secretion, and axonal regeneration of dorsal root ganglia (DRG) were evaluated by coculture with nerve microtis sues and PRP. In vivo, seventy-five rabbits were equally and randomly divided into Hollow, PRP, Micro-T (Microtissues), Micro-T + PRP and Autograft groups. By analysing the neurological function, electrophysiological recovery, and the comparative results of multimodal ultrasound and histological evaluation, we investigated the effect of these new nerve grafts in repairing tibial nerve defects. Results: Our results showed that the combined application of nerve microtissues and PRP could significantly promote the proliferation, secretion and migration of SCs and the regeneration of axons in the early stage. The Micro-T + PRP group and Autograft groups exhibited the best nerve repair 12 weeks postoperatively. In addition, the changes in target tissue stiffness and microvascular perfusion on multimodal ultrasound (shear wave elastography; contrast-enhanced ultrasonography; Angio PlaneWave UltrasenSitive, AngioPLUS) were significantly correlated with the histological results, such as collagen area percentage and VEGF expression, respectively. Conclusion: Our novel tissue-engineered nerve graft shows excellent efficacy in repairing 12-mm defects of the tibial nerve in rabbits. Moreover, multimodal ultrasound may provide a clinical reference for prognosis by quantita tively evaluating the stiffness and microvescular flow of nerve grafts and targeted muscles
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A case report of a long-term abandoned torn lingual nerve injury repaired by collagen nerve graft induced by lower third molar extraction
Maxillofacial Plastic Reconstructive Surgery
2019 .01
-
Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration
Journal of dental anesthesia and pain medicine
2017 .01
-
소아 급성림프구성백혈병에서 항암화학요법 후 발생하는 말초신경병증의 양상
Clinical Pediatric Hematology-Oncology
2017 .01
-
회돌이 후두신경재생을 위한 신경도관의 활용
International Journal of Thyroidology
2023 .05
-
신경 신장 적용 시간에 따른 신경흥분성 변화
PNF and movement
2018 .01
-
Using ultrasound shear wave elastography to characterize peripheral nerve mechanics: a systematic review on the normative reference values in healthy individuals
ULTRASONOGRAPHY
2024 .05
-
Fabrication Techniques of Nerve Guidance Conduits for Nerve Regeneration
Yonsei Medical Journal
2022 .02
-
Optical Biopsy of Peripheral Nerve Using Confocal Laser Endomicroscopy: A New Tool for Nerve Surgeons?
Archives of plastic surgery : APS
2015 .01
-
Reconstruction of the sciatic nerve using bilateral vascularized sural nerve grafts: a case report
Archives of Hand and Microsurgery
2022 .03
-
Complex Variations of the Sciatic Nerve Branch and Morphological Variation of the Piriformis Muscle: a Case Report
해부·생물인류학
2022 .12
-
Multivariable Analysis in Recovery of Mandibular Nerve Disturbance
Journal of Korean Dental Science
2025 .03
-
Sciatic neurotmesis and periostitis ossificans progressiva due to a traumatic/unexpected glass injury: a case report
Journal of Yeungnam Medical Science
2024 .01
-
Ultrasonography Evaluation of Vulnerable Vessels Around Cervical Nerve Roots During Selective Cervical Nerve Root Block
Annals of Rehabilitation Medicine
2017 .01
-
외상환자에서의 초음파 유도 하지 국소 신경차단술의 유용성
Journal of trauma and injury : JTI
2015 .01
-
다발신경병 진단에서 신경초음파검사
대한근전도 전기진단의학회지
2019 .01
-
Usefulness of Ultrasound-Guided Lower Extremity Nerve Blockade in Surgery for Patellar Fracture
Knee Surgery and Related Research
2015 .01
-
Long-Term Follow-Up after the Sural Nerve Graft on the Injured Temporal Branch of the Facial Nerve: A Case Report
Archives of Hand and Microsurgery
2018 .01
-
Branching Patterns of Medial and Inferior Calcaneal Nerves Around the Tarsal Tunnel
Annals of Rehabilitation Medicine
2015 .01
-
Diagnosis and treatment of nerve injury following venipuncture - A report of two cases -
Anesthesia and Pain Medicine
2021 .01
-
Risk of Encountering Dorsal Scapular and Long Thoracic Nerves during Ultrasound-guided Interscalene Brachial Plexus Block with Nerve Stimulator
The Korean Journal of Pain
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0