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논문 기본 정보

자료유형
학술저널
저자정보
우승희 (경희대학교) Kim Yoo Rim (Department of Physiology Seoul National University College of Medicine Seoul 03080 KoreaNeuroscienc) Bak Myeong Seong (Kyung Hee University) Chung Geehoon (Kyung Hee University) Kim Sang Jeong (Seoul National University College of Medicine) Kim Sun Kwang (Kyung Hee University)
저널정보
한국뇌신경과학회 Experimental Neurobiology Experimental Neurobiology Vol.31 No.5
발행연도
2022.10
수록면
324 - 331 (8page)
DOI
10.5607/en22029

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초록· 키워드

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Itch and pain are distinct sensations that share anatomically similar pathways: from the periphery to the brain. Over the last decades, several itchspecific neural pathways and molecular markers have been identified at the peripheral and spinal cord levels. Although the perception of sensation is ultimately generated at the brain level, how the brain separately processes the signals is unclear. The primary somatosensory cortex (S1) plays a crucial role in the perception of somatosensory information, including touch, itch, and pain. In this study, we investigated how S1 neurons represent itch and pain differently. First, we established a spontaneous itch and pain mouse model. Spontaneous itch or pain was induced by intradermal treatment with 5-HT or capsaicin on the lateral neck and confirmed by a selective increase in scratching or wiping-like behavior, respectively. Next, in vivo two-photon calcium imaging was performed in awake mice after four different treatments, including 5-HT, capsaicin, and each vehicle. By comparing the calcium activity acquired during different sessions, we distinguished the cells responsive to itch or pain sensations. Of the total responsive cells, 11% were both responsive, and their activity in the pain session was slightly higher than that in the itch session. Itch- and painpreferred cells accounted for 28.4% and 60.6%, respectively, and the preferred cells showed the lowest activity in their counter sessions. Therefore, our results suggest that S1 uses a multiplexed coding strategy to encode itch and pain, and S1 neurons represent the interaction between itch and pain.

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