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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.11
- 수록면
- 851 - 860 (10page)
- DOI
- 10.14348/molcells.2021.0190
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초록· 키워드
오류제보하기
Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells?namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell?based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.
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참고문헌 (43)
참고문헌 신청
Al-Bagdadi, F. A. / 2019 / Ultrastructure morphological characterization of different passages of rat dental follicle stem cells at in vitro culture / J. Microsc. Ultrastruct 7:57~64
Barer, R. / 1953 / Determination of dry mass, thickness, solid and water concentration in living cells / Nature 172:1097~1098
Boulon, S. / 2010 / The nucleolus under stress / Mol. Cell 40:216~227
최문환 / 2014 / Therapeutic Use of Stem Cell Transplantation for Cell Replacement or Cytoprotective Effect of Microvesicle Released from Mesenchymal Stem Cell / Molecules and Cells 37(2):133~139
Debnath, S.K. / 2011 / Real-time quantitative phase imaging with a spatial phase-shifting algorithm / Opt. Lett. 36:4677~4679
Barer, R. / 1953 / Determination of dry mass, thickness, solid and water concentration in living cells / Nature 172:1097~1098
Boulon, S. / 2010 / The nucleolus under stress / Mol. Cell 40:216~227
최문환 / 2014 / Therapeutic Use of Stem Cell Transplantation for Cell Replacement or Cytoprotective Effect of Microvesicle Released from Mesenchymal Stem Cell / Molecules and Cells 37(2):133~139
Debnath, S.K. / 2011 / Real-time quantitative phase imaging with a spatial phase-shifting algorithm / Opt. Lett. 36:4677~4679
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