지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제11호
- 발행연도
- 2021.11
- 수록면
- 1,481 - 1,489 (9page)
- DOI
- 10.4014/jmb.2103.03044
이용수
초록· 키워드
오류제보하기
Early and accurate detection of pathogens is important to improve clinical outcomes of bloodstream infections (BSI), especially in the case of drug-resistant pathogens. In this study, we aimed to develop a culture-independent digital PCR (dPCR) system for multiplex detection of major sepsiscausing gram-negative pathogens and antimicrobial resistance genes using plasma DNA from BSI patients. Our duplex dPCR system successfully detected nine targets (five bacteria-specific targets and four antimicrobial resistance genes) through five reactions within 3 hours. The minimum detection limit was 50 ag of bacterial DNA, suggesting that 1 CFU/ml of bacteria in the blood can be detected. To validate the clinical applicability, cell-free DNA samples from febrile patients were tested with our system and confirmed high consistency with conventional blood culture. This system can support early identification of some drug-resistant gram-negative pathogens, which can help improving treatment outcomes of BSI.
목차
등록된 정보가 없습니다.
참고문헌 (41)
참고문헌 신청
Itokazu GS / 1996 / Antimicrobial resistance rates among aerobic gram-negative bacilli recovered from patients in intensive care units : evaluation of a national postmarketing surveillance program / Clin. Infect. Dis 23:779~784
System N / 1999 / National Nosocomial Infections Surveillance (NNIS) system report, data summary from January 1990-May 1999, issued June 1999. A report from the NNIS system / Am. J. Infect. Control 27:520~532
Ling TK / 2006 / Multicenter antimicrobial susceptibility survey of gram-negative bacteria isolated from patients with community-acquired infections in the People's Republic of China / Antimicrob. Agents Chemother 50:374~378
Talbot GH / 2006 / Bad bugs need drugs : an update on the development pipeline from the antimicrobial availability task force of the infectious diseases society of America / Clin. Infect. Dis 42:657~668
Kollef MH / 2000 / Inadequate antimicrobial treatment : an important determinant of outcome for hospitalized patients / Clin. Infect. Dis 31(Suppl 4):S131~138
System N / 1999 / National Nosocomial Infections Surveillance (NNIS) system report, data summary from January 1990-May 1999, issued June 1999. A report from the NNIS system / Am. J. Infect. Control 27:520~532
Ling TK / 2006 / Multicenter antimicrobial susceptibility survey of gram-negative bacteria isolated from patients with community-acquired infections in the People's Republic of China / Antimicrob. Agents Chemother 50:374~378
Talbot GH / 2006 / Bad bugs need drugs : an update on the development pipeline from the antimicrobial availability task force of the infectious diseases society of America / Clin. Infect. Dis 42:657~668
Kollef MH / 2000 / Inadequate antimicrobial treatment : an important determinant of outcome for hospitalized patients / Clin. Infect. Dis 31(Suppl 4):S131~138
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