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논문 기본 정보
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- 학술저널
- 저자정보
- 발행연도
- 2017.7
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Background: This study was performed to investigate the impact of exogenous ghrelin on the pancreatic α-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine.
Methods: Sprague-Dawley male rats (9 weeks old, 300 ± 10 g) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and 10.0 μg/kg body weight (BW), respectively. The plasma ghrelin and cholecystokinin (CCK) level were determined using enzyme immunoassay kit; the mRNA expression of ghrelin receptor (GHSR-1α) and growth hormone (GH) receptor were assessed by reverse transcription PCR; the expressions of pancreatic α-amylase activity, extracellular-signal-regulated kinases (ERK), phosphorylated extracellular-signal-regulated kinases (pERK) and c-Jun N-terminal kinase (JNK) were evaluated by western blotting; moreover the responses of pancreatic proteins to ghrelin were analyzed using the two-dimensional gel electrophoresis system.
Results: The exogenous ghrelin (1.0 and 10.0 μg/kg BW) elevated the level of plasma ghrelin (p < 0.05), and suppressed the expression of pancreatic α-amylase at a dose of 10.0 μg/kg BW (p < 0.05). No difference in the level of plasma CCK was observed, even though rats were exposed to any dose of exogenous ghrelin. In addition, a combination of western blot and proteomic analysis revealed exogenous ghrelin (10.0 μg/kg BW) induced increasing the JNK and ERK expressions (p < 0.05) and four proteins such as Destrin, Anionic trypsin-1, Trypsinogen, and especially eukaryotic translation initiation factor 3 in rat pancreas.
Conclusions: Taken together, exogenous ghrelin by i.p. infusion plays a role in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling pathway.
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참고문헌 (16)
참고문헌 신청
Gualillo O / 2003 / Ghrelin, a widespread hormone : insights into molecular and cellular regulation of its expression and mechanism of action / FEBS Lett 552:105~109
Kojima M / 2002 / Ghrelin, an orexigenic signaling molecule from the gastrointestinal tract / Curr Opin Pharmacol 2:665~668
Zhang W / 2001 / Inhibition of pancreatic protein secretion by ghrelin in the rat / J Physiol 537:231~236
Sato N / 2003 / Central administration of ghrelin stimulates pancreatic exocrine secretion via the vagus in conscious rats / The Jpn J Physiol 53:443~449
Li Y / 2006 / Ghrelin acts on the dorsal vagal complex to stimulate pancreatic protein secretion / Am J Physiol Gastrointest Liver Physiol 290:G1350~G1358
Kojima M / 2002 / Ghrelin, an orexigenic signaling molecule from the gastrointestinal tract / Curr Opin Pharmacol 2:665~668
Zhang W / 2001 / Inhibition of pancreatic protein secretion by ghrelin in the rat / J Physiol 537:231~236
Sato N / 2003 / Central administration of ghrelin stimulates pancreatic exocrine secretion via the vagus in conscious rats / The Jpn J Physiol 53:443~449
Li Y / 2006 / Ghrelin acts on the dorsal vagal complex to stimulate pancreatic protein secretion / Am J Physiol Gastrointest Liver Physiol 290:G1350~G1358
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