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논문 기본 정보

자료유형
학술저널
저자정보
Lee Haeun (Dankook University) Jeong Jinuk (Dankook University) Oh Yunseok (Dankook University) Lee Cherl-Jun (Dankook University) Mun Seyoung (Dankook University) Lee Dong-Geol (R&I Center COSMAX BTI) Jo HyungWoo (R&I Center COSMAX BTI) Heo Young Mok (R&I Center COSMAX BTI) Baek Chaeyun (R&I Center COSMAX BTI) Heo Chan Yeong (Seoul National University Bundang Hospital) Kang So Min (Seoul National University Bundang Hospital) 한규동 (단국대학교)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.43 No.12
발행연도
2021.12
수록면
1,483 - 1,495 (13page)
DOI
10.1007/s13258-021-01180-2

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Background Skin is an essential outer barrier and supports the growth of commensal microorganisms that protects a host from the ofense of foreign toxic organisms. With the rapid development of next-generation sequencing (NGS)-based applications, skin microbiome research for facial health care has reached industry growth, such as therapy and cosmetic product development. Despite the acceleration of skin microbiome research, experimental standardization protocol has not yet been established in the facial site and method of sampling. Objective Thus, we aimed to investigate the diferences in microbial composition at each facial site (cheek, mouth, forehead, and entire face) using comprehensive microbiome analysis. Methods Twelve specimens from three men (four specimens per one person) were collected. The hypervariable regions (V3?V4) of the bacterial 16S rRNA gene were targeted for 16S amplicon library construction and classifcation of bacterial taxonomy. Skin microbial composition for all specimens was investigated, and the diferences site-by-site in skin microbial composition were analyzed and evaluated by the various statistical tests. Results We were able to validate the independent correlation between the skin microbiome composition and the facial sites. The cheek site showed the highest alpha-diversity in richness and evenness scores compared to the forehead and mouth. The cheek and mouth sites showed a positive correlation (R2 value>0.93) with the entire face, while the forehead sites were negatively correlated (R2 value<0.2). Given the relative abundance based on statistical correlation analysis, we estimated that the cheek site could be considered an optimal topical site to replace the entire face. Conclusion Our study suggests that skin microbiome profling of four facial sites confrms that the cheek shows the most similar skin fora with the entire face. This study would be informative for preventing bias caused by sampling methods before researching and understanding skin cosmetics development or skin diseases.

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