지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 1,035 - 1,044 (10page)
- DOI
- 10.1007/s13770-021-00383-8
이용수
초록· 키워드
오류제보하기
Background: Impaired potential of hypoxia-mediated angiogenesis lead poor healing of diabetic wounds. Previous studies have shown that extracellular vesicles from adipose derived stem cells (ADSC-EVs) accelerate wound healing with unelucidated mechanism. However, it is not yet clear about the underlying mechanism of ADSC-EVs in regulating the hypoxia-related PI3K/AKT/mTOR signaling pathway of vascular endothelial cells in diabetic wounds. Therefore, in this study, human derived ADSC-EVs (hADSC-EVs) isolated from adipose tissue were co-cultured with advanced glycosylation end product (AGE) treated human umbilical vein endothelial cells (HUVECs) in vitro and local injected into the wounds of diabetic rats.
Methods: In vitro, the therapeutic potential of hADSC-EVs on AGE-treated HUVECs was evaluated by cell counting kit-8, scratching, and tube formation assay. Subsequently, the effects of hADSC-EVs on the PI3K/AKT/mTOR/HIF-1α signaling pathway were also assayed by qRT-PCR and western blot. In vivo, the effect of hADSC-EVs on diabetic wound healing in rats were also assayed by closure kinetics, Masson staining and HIF-1α-CD31 immunofluorescence.
Results: hADSC-EVs were spherical in shape with an average particle size of 198.1?±?91.5 nm, and were positive for CD63, CD9 and TSG101. hADSC-EVs promoted the expression of PI3K-AKT-mTOR-HIF-1α signaling pathway of AGEs treated HUVECs with improved the potential of proliferation, migration and tube formation, and improve the healing and angiogenesis of diabetic wound in rats. However, the effect of hADSC-EVs described above can be blocked by PI3K-AKT inhibitor both in vitro and vivo.
Conclusion: Our findings indicated that hADSC-EVs accolated the healing of diabetic wounds by promoting HIF-1α-mediated angiogenesis in the PI3K-AKT-mTOR depend manner.
목차
등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
International Diabetes Federation / IDF Diabetes Atlas 9th Edition, Global Estimates for the Prevalence of Diabetes for 2019, 2030 and 2045
Thangarajah H / 2009 / The molecular basis for impaired hypoxia-induced VEGF expression in diabetic tissues / Proc Natl Acad Sci U S A 106:13505~13510
Tandara AA / 2004 / Oxygen in wound healing-more than a nutrient / World J Surg 28:294~300
Covello KL / 2004 / HIFs, hypoxia, and vascular development / Curr Top Dev Biol 62:37~54
Ceradini DJ / 2004 / Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 / Nat Med 10:858~864
Thangarajah H / 2009 / The molecular basis for impaired hypoxia-induced VEGF expression in diabetic tissues / Proc Natl Acad Sci U S A 106:13505~13510
Tandara AA / 2004 / Oxygen in wound healing-more than a nutrient / World J Surg 28:294~300
Covello KL / 2004 / HIFs, hypoxia, and vascular development / Curr Top Dev Biol 62:37~54
Ceradini DJ / 2004 / Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 / Nat Med 10:858~864
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