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논문 기본 정보

자료유형
학술저널
저자정보
Gour Gobindo Das (Department of Genetics and Plant Breeding Bangladesh Agricultural University Mymensingh 2202 Bangla) Md Abdul Malek (Plant Breeding Division Bangladesh Institute of Nuclear Agriculture Mymensingh 2202 Bangladesh) AKM Shamsuddin (Department of Genetics and Plant Breeding Bangladesh Agricultural University Mymensingh 2202 Bangla) GHM Sagor (Plant Breeding Division Bangladesh Institute of Nuclear Agriculture Mymensingh 2202 Bangladesh)
저널정보
한국육종학회 Plant Breeding and Biotechnology Plant Breeding and Biotechnology Vol.9 No.3
발행연도
2021.9
수록면
171 - 184 (14page)

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초록· 키워드

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Synthetic B. napus was produced through interspecific hybridization between nine varieties of B. rapa and only one exotic variety of B. oleracea var. alboglabra along with exogenous application of gibberellic acid (GA3) before pollination. A total of eighteen crosses including their reciprocals were made between the two species. Crossability in both way directions between the two species of Brassica was not equally success. The degree of success was significantly influenced by maternal genotypes. On average, the cross success was 8.42% when the varieties of B. rapa used as female parents in contrast to 2.88% when B. rapa used a pollen parents. Among the four concentrations (25, 50, 75 and 100 ppm) of GA3, 75 ppm gave highest response for different crossability characters in both way cross directions. The hybrids contained 19 somatic chromosomes which were the sum of the gametic chromosome number of B. rapa and B. oleracea. Of the two methods, followed to induce chromosome doubling in the adult plants, the Modified Injection Method was found more effective than the Cotton Plug Method. Among different concentration of colchinine 0.20% gave the highest success (66.67%) of chromosome doubling in the hybrids. All the colchiploid (C1) plants contained 38 chromosomes in their somatic cells which were the sum of the somatic chromosomes of both species. The genomes of resynthesized lines were also identified through Brassica genome specific SSR markers. The presence of markers for both A and C genome was detected in resynthesized lines suggesting that their genomic constitution was AACC.

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