Inhibitory effect of StCYP707A1 gene on tuberization in transgenic potato

Lu Liu; Rui-Jie Zhang; Wen-jiao Zhu; Xin-Ru Liu; Ke Shi; Min Chen; Qing Yang

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자료유형: 학술저널

저자정보: Lu Liu (Nanjing Agricultural University) Rui-Jie Zhang (Nanjing Agricultural University) Wen-jiao Zhu (Nanjing Agricultural University) Xin-Ru Liu (Nanjing Agricultural University) Ke Shi (Nanjing Agricultural University) Min Chen (Nanjing Agricultural University) Qing Yang (Nanjing Agricultural University)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제11권 제4호

발행연도: 2017.8

수록면: 219 - 228 (10page)

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연구배경

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Tuber formation of potato is a complex biological process and is regulated by many factors including phytochrome, hormones, transcription factors, RNAs, microRNAs, etc. In our previous study, CYP707A1, an ABA 80-hydroxylase gene, was down-regulated in the StCOL antisense transgenic potato stolons, but its relation with potato tuberization has not been studied yet. In this study, to investigate the role of this gene in potato tuberization, we cloned a CYP707A1 gene from potato cultivar De ´sire ´e and constructed the StCYP707A1 over-expression and anti-sense potato lines by Agrobacterium-mediated transformation. In total, four over-expression transgenic lines and nine anti-sense transgenic lines were con?rmed by PCR analysis. Semi-quantitative RT-PCR and qRTPCR analysis showed that the level of StCYP707A1 transcripts was signi?cantly higher in the over-expression lines (A7 and A9) and lower in the anti-sense lines (F2 and F8), compared to the wild-type control. We further analyzed tuber formation in the transgenic lines and the wild-type control. The result showed that tuber yield per plant and average tuber weight were decreased in A7 and A9 and increased in F2. We also measured the content of ABA and GA3 in transgenic lines. ABA level was reduced in A7 and A9 and increased in F2. Contrariwise, the concentration of GA3 was higher in A7 and A9, and lower in F2 than in wild-type control. These results indicate that StCYP707A1 negatively affects potato tuberization through ABA regulation on gibberellic acid concentration.

#Potato #StCYP707A1 #Tuberization #Regulation #ABA

참고문헌 (28)

참고문헌 신청

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