Overexpressing fusion proteins of 4-coumaroyl-CoA ligase (4CL) and stilbene synthase (STS) in tobacco plants leading to resveratrol accumulation and improved stress tolerance

Xuancheng He; Feiyan Xue; Lulu Zhang; Hui-Li Guo; Lan-Qing Ma; Mingfeng Yang

추천

검색

자료유형: 학술저널

저자정보: Xuancheng He (Beijing University of Agriculture) Feiyan Xue (Beijing University of Agriculture) Lulu Zhang (Beijing University of Agriculture) Hui-Li Guo (Beijing University of Agriculture) Lan-Qing Ma (Beijing University of Agriculture) Mingfeng Yang (Beijing University of Agriculture)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제12권 제5호

발행연도: 2018.10

수록면: 295 - 302 (8page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Resveratrol (trans-3,4′,5-trihydroxystilbene) is a phytoalexin produced in plants in response to pathogen attack as a part of plant defense response and it is also a highly bioactive substance of pharmaceutical interest. To obtain transgenic plants with a high level of resveratrol, two enzymes in the last two steps of resveratrol synthesis, 4-coumaroyl-CoA ligase (4CL) and stilbene synthase (STS), were fused together by a glycine?serine?glycine (GSG) tripeptide linker, and the 4CL-GSGSTS construct driven by a CaMV35S promoter was transformed into tobacco (Nicotiana benthamiana) by Agrobacteriummediated method. In the transgenic plants, a high resveratrol level was detected (21.05 μg/g fresh weight) by high-pressure liquid chromatography (HPLC), which is higher than previous transgenic plants with only STS gene overexpression. In addition to resistance to pathogen, transgenic plants showed improved tolerance to salt and osmotic stresses, and the lower level of malondialdehyde (MDA) in transgenic plants suggested that resveratrol could protect plant membrane lipid from peroxidation under abiotic stresses.

#4-Coumaroyl-CoA ligase (4CL) · Stilbene synthase (STS) · Resveratrol · Stress tolerance · Fusion proteins

참고문헌 (51)

참고문헌 신청

Aleynova OA / 2016 / Stilbene accumulation in cell cultures of Vitis amurensis Rupr. overexpressing VaSTS1. VaSTS2, and VaSTS7 genes / Plant Cell Tiss Org 125:329~339

Bell MR / 2013 / To fuse or not to fuse : what is your purpose? / Protein Sci 22:1466~1477

Bostanghadiri N / 2017 / Comprehensive review on the antimicrobial potency of the plant polyphenol resveratrol / Biomed Pharmacother 95:1588~1595

Bulow L / 1990 / Preparation of artificial bifunctional enzymes by gene fusion / Biochem Soc Symp 57:123~133

Cheng S / 2016 / Genetic transformation of a fruit-specific, highly expressed stilbene synthase gene from Chinese wild Vitis quinquangularis / Planta 243:1041~1053

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!